Tested Applications
| Positive WB detected in | Neuro-2a cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24313-1-AP targets LYSMD3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19430 Product name: Recombinant human LYSMD3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-142 aa of BC136741 Sequence: MAGRHQNRSFPLPGVQSSGQVHAFGNCSDSDILEEDAEVYELRSRGKEKVRRSTSRDRLDDIIVLTKDIQEGDTLNAIALQYCCTVADIKRVNNLISDQDFFALRSIKIPVKKFSSLTETLCPPKGRQTSRHSSVQYSSEQQ Predict reactive species |
| Full Name | LysM, putative peptidoglycan-binding, domain containing 3 |
| Calculated Molecular Weight | 306 aa, 35 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC136741 |
| Gene Symbol | LYSMD3 |
| Gene ID (NCBI) | 116068 |
| RRID | AB_2879490 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z3D4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LYSMD3 also termed as LysM and putative peptidoglycan binding domain containing protein 3 is a 306 amino acid single-pass membrane protein that contains one LysM repeat. LysM domain encoding genes may play a role in brain and in nervous system development and functioning. LYSMD3, shows a high level of central nervous expression during embryogenesis and is also, therefore, a good candidate gene for other central nervous system (CNS) symptoms, such as psychomotor retardation, brain anomalies and muscular hypotonia of the 5q14.3 microdeletion syndrome.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for LYSMD3 antibody 24313-1-AP | Download protocol |
| WB protocol for LYSMD3 antibody 24313-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LYSMD3 Polyclonal antibody (Cat# 24313-1-AP) has 3 citations published in Cell Reports, Journal of Biological Chemistry, and Molecular Biology of the Cell. The cited research spans mammalian pattern recognition (chitin sensing), type II membrane protein biology, and Golgi-specific transcriptional stress response. All three citations used the antibody for Western blot in human samples.
| Species | Application | Title |
|---|---|---|
J Biol Chem LysMD3 is a type II membrane protein without an in vivo role in the response to a range of pathogens. | ||
Mol Biol Cell Targeted protein unfolding uncovers a Golgi-specific transcriptional stress response. |
