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LZIC Polyclonal antibody

LZIC Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14543-1-AP

Synonyms

LZIC, Protein LZIC

Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using LZIC Polyclonal antibody (14543-1-AP)

    WB analysis of BxPC-3 using 14543-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14543-1-AP (LZIC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using LZIC Polyclonal antibody (14543-1-AP)

    WB analysis of BxPC-3 using 14543-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14543-1-AP (LZIC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using LZIC Polyclonal antibody (14543-1-AP)

    WB analysis of HepG2 using 14543-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14543-1-AP (LZIC antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using LZIC Polyclonal antibody (14543-1-AP)

    WB analysis of HEK-293 using 14543-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14543-1-AP (LZIC antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inBxPC-3 cells, HepG2 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14543-1-AP targets LZIC in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LZIC fusion protein Ag6047
Full Name leucine zipper and CTNNBIP1 domain containing
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21-24 kDa
GenBank Accession NumberBC063443
Gene Symbol LZIC
Gene ID (NCBI) 84328
RRIDAB_2138923
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LZIC is closely related to ICAT, a physiological inhibitor of the Wnt pathway that interacts physically and functionally with β-catenin to prevent the transcription of Wnt target genes. LZIC's ICAT-homologous region is highly similar to ICAT with particular conservation of residues that are used by ICAT for β-catenin-binding. LZIC does not interact with β-catenin in vitro or in vivo. LZIC is a protein conserved in vertebrates, is required for neuronal survival.

Protocols

Product Specific Protocols
WB protocol for LZIC antibody 14543-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols