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  • KD/KO Validated

LZTFL1 Polyclonal antibody

LZTFL1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17073-1-AP

Synonyms

LZTFL1



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, U-251 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman heart tissue, human lung tissue, human colon tissue, human testis tissue, mouse heart tissue, mouse lung tissue, mouse small intestine tissue, mouse testis tissue, rat heart tissue, rat small intestine tissue, rat testis tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17073-1-AP targets LZTFL1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LZTFL1 fusion protein Ag10919
Full Name leucine zipper transcription factor-like 1
Calculated Molecular Weight 299 aa, 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC025988
Gene Symbol LZTFL1
Gene ID (NCBI) 54585
RRIDAB_10950400
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LZTFL1 belongs to the LZTFL1 family. It is an important regulator of BBSome ciliary trafficking and hedgehog signaling. LZTFL1 regulates Ciliary Trafficking of the BBSome and Smoothened.(PMID:22072986)

Protocols

Product Specific Protocols
WB protocol for LZTFL1 antibody 17073-1-APDownload protocol
IHC protocol for LZTFL1 antibody 17073-1-APDownload protocol
IF protocol for LZTFL1 antibody 17073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Dev Cell

IFT27 Links the BBSome to IFT for Maintenance of the Ciliary Signaling Compartment.

Authors - Thibaut Eguether
  • KO Validated
mouseWB,IF

EMBO J

Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.

Authors - Shichao Duan
mouseIF

J Cell Biol

Ubiquitin links smoothened to intraflagellar transport to regulate Hedgehog signaling.

Authors - Paurav B Desai
humanWB,IHC,IP

Oncogene

LZTFL1 inhibits kidney tumor cell growth by destabilizing AKT through ZNRF1-mediated ubiquitin proteosome pathway

Authors - Jun Lu