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  • Featured Product
  • KD/KO Validated

LZTS1 Polyclonal antibody

LZTS1 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 20878-1-AP

Synonyms

F37, FEZ1, LZTS1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LZTS1 Polyclonal antibody (20878-1-AP)

    WB analysis using 20878-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using LZTS1 Polyclonal antibody (20878-1-AP)

    WB analysis of PC-3 using 20878-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using LZTS1 Polyclonal antibody (20878-1-AP)

    WB analysis of mouse thymus using 20878-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using LZTS1 Polyclonal antibody (20878-1-AP)

    WB analysis of HEK-293 using 20878-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using LZTS1 Polyclonal antibody (20878-1-AP)

    WB analysis of mouse ovary using 20878-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using LZTS1 Polyclonal antibody (20878-1-AP)

    WB analysis of human testis using 20878-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using LZTS1 Polyclonal antibody (20878-1-AP)

    WB analysis of Jurkat using 20878-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using LZTS1 Polyclonal antibody (20878-1-AP)

    IP experiment of mouse brain using 20878-1-AP

    IP result of anti-LZTS1 (IP:20878-1-AP, 3ug; Detection:20878-1-AP 1:500) with mouse brain tissue lysate 7500ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LZTS1 Polyclonal antibody (20878-1-AP)

    IF Staining of HepG2 using 20878-1-AP

    Immunofluorescent analysis of HepG2 cells, using LZTS1 antibody 20878-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, human testis tissue, mouse ovary tissue, mouse thymus tissue, PC-3 cells, rat brain tissue, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20878-1-AP is LZTS1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LZTS1 fusion protein Ag14977
Full Name leucine zipper, putative tumor suppressor 1
Calculated Molecular Weight 596 aa, 67 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC075007
Gene Symbol LZTS1
Gene ID (NCBI) 11178
RRIDAB_10693683
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LZTS1 antibody 20878-1-APDownload protocol
IF protocol for LZTS1 antibody 20878-1-APDownload protocol
IP protocol for LZTS1 antibody 20878-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Nat Commun

Lzts1 controls both neuronal delamination and outer radial glial-like cell generation during mammalian cerebral development.

Authors - T Kawaue
  • KO Validated
View Article
humanWB

Mol Oncol

Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis

Authors - Tianwei Chen
View Article