Filter: AllWBIPIHCIF/ICC

WB analysis using 20878-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 20878-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 20878-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 20878-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 20878-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 20878-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse ovary using 20878-1-AP
mouse ovary tissue were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 20878-1-AP
IP result of anti-LZTS1 (IP:20878-1-AP, 3ug; Detection:20878-1-AP 1:500) with mouse brain tissue lysate 7500ug.
IHC staining of human skin cancer using 20878-1-AP
Immunohistochemical analysis of paraffin-embedded skin cancer slide using 20878-1-AP (LZTS1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 20878-1-AP
Immunofluorescent analysis of HepG2 cells, using LZTS1 antibody 20878-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Featured Product
KD/KO Validated

LZTS1 Polyclonal antibody

Name: LZTS1 Polyclonal antibody
Brand: Proteintech
SKU: 20878-1-AP

LZTS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 20878-1-AP

Publications(3)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

F37, F37/esophageal cancer-related gene-coding leucine-zipper motif, Fez1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information
Publications (3)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells, HEK-293 cells, human testis tissue, mouse ovary tissue, mouse thymus tissue, PC-3 cells, rat brain tissue, mouse brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human skin cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

20878-1-AP targets LZTS1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag14977 Product name: Recombinant human LZTS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 397-596 aa of BC075007 Sequence: QLKESQTEVNAKASEILGLKAQLKDTRGKLEGLELRTQDLEGALRTKGLELEVCENELQRKKNEAELLREKVNLLEQELQELRAQAALARDMGPPTFPEDVPALQRELERLRAELREERQGHDQMSSGFQHERLVWKEEKEKVIQYQKQLQQSYVAMYQRNQRLEKALQQLARGDSAGEPLEVDLEGADIPYEDIIATEI Predict reactive species
Full Name	leucine zipper, putative tumor suppressor 1
Calculated Molecular Weight	596 aa, 67 kDa
Observed Molecular Weight	67 kDa
GenBank Accession Number	BC075007
Gene Symbol	LZTS1
Gene ID (NCBI)	11178
RRID	AB_10693683
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y250
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LZTS1 (Leucine Zipper Tumor Suppressor 1), also known as FEZ1, is a tumor suppressor protein that plays a critical role in regulating cell cycle progression and maintaining microtubule stability. It is frequently downregulated in various human cancers, including breast, gastric, and prostate cancer. LZTS1 interacts with the anaphase-promoting complex/cyclosome (APC/C) to promote the degradation of key mitotic regulators such as cyclin B1, thereby controlling the G2/M transition. Additionally, it influences cytoskeletal dynamics by binding to microtubules and actin filaments, affecting cell adhesion, migration, and proliferation. Loss or reduced expression of LZTS1 is associated with aggressive tumor behavior, increased metastatic potential, and poor clinical prognosis, highlighting its importance as a potential biomarker and therapeutic target in oncology.

Protocols

Product Specific Protocols
IF protocol for LZTS1 antibody 20878-1-AP	Download protocol
IHC protocol for LZTS1 antibody 20878-1-AP	Download protocol
IP protocol for LZTS1 antibody 20878-1-AP	Download protocol
WB protocol for LZTS1 antibody 20878-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IF	Nat Commun Lzts1 controls both neuronal delamination and outer radial glial-like cell generation during mammalian cerebral development. Authors - T Kawaue KO Validated View Article
human	WB	Mol Oncol Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis Authors - Tianwei Chen View Article
human	WB,IHC	Int Dent J Screening Differentially Expressed Proteins in Areca Nut-Related Oral Squamous Cell Carcinoma Using Tandem Mass Tag Proteomics Authors - Xiaoqun Mai View Article