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KD/KO Validated

MAEL Polyclonal antibody

MAEL Polyclonal Antibody for WB, IHC, ELISA

Cat No. 26666-1-AP

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, RIP, ELISA

Synonyms

MAEL, maelstrom homolog (Drosophila), Protein maelstrom homolog, RP11 102C16.1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Filter: AllWBIHC

WB analysis of rat testis using 26666-1-AP
rat testis tissue were subjected to SDS PAGE followed by western blot with 26666-1-AP (MAEL Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse testis using 26666-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 26666-1-AP (MAEL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 26666-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26666-1-AP (MAEL Antibody) at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 26666-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26666-1-AP (MAEL Antibody) at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse testis using 26666-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26666-1-AP (MAEL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (2)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	rat testis tissue, mouse testis tissue
Positive IHC detected in	human testis tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26666-1-AP targets MAEL in WB, IHC, IF, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag24524 Product name: Recombinant human MAEL protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 282-434 aa of BC028595 Sequence: RCKWHEENDILFCALAVCKKIAYCISNSLATLFGIQLTEAHVPLQDYEASNSVTPKMVVLDAGRYQKLRVGSSGFSHFNSSNEEQRSNTPIGDYPSRAKISGQNSSVRGRGITRLLESISNSSSNIHKFSNCDTSLSPYMSQKDGYKSFSSLS Predict reactive species
Full Name	maelstrom homolog (Drosophila)
Calculated Molecular Weight	434 aa, 49 kDa
Observed Molecular Weight	46-50 kDa
GenBank Accession Number	BC028595
Gene Symbol	MAEL
Gene ID (NCBI)	84944
RRID	AB_2880595
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96JY0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for MAEL antibody 26666-1-AP	Download protocol
WB protocol for MAEL antibody 26666-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	WB,IHC,IF,RIP	Andrology The MAEL expression in mitochondria of human spermatozoa and the association with asthenozoospermia Authors - Yu-Sheng Cheng KD Validated View Article
mouse	WB	J Cell Mol Med Loss-of-function variant in TDRD6 cause male infertility with severe oligo-astheno-teratozoospermia in human and mice Authors - Xinying Bi View Article