Tested Applications
| Positive WB detected in | human lung tissue |
| Positive IP detected in | SKOV-3 cells |
| Positive IHC detected in | human ovary tumor tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 13 publications below |
| IHC | See 4 publications below |
| IF | See 5 publications below |
| CoIP | See 1 publications below |
Product Information
12771-1-AP targets MAFF in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3460 Product name: Recombinant human MAFF protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-164 aa of BC015037 Sequence: MSVDPLSSKALKIKRELSENTPHLSDEALMGLSVRELNRHLRGLSAEEVTRLKQRRRTLKNRGYAASCRVKRVCQKEELQKQKSELEREVDKLARENAAMRLELDALRGKCEALQGFARSVAAARGPATLVAPASVITIVKSTPGSGSGPAHGPDPAHGPASCS Predict reactive species |
| Full Name | v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian) |
| Calculated Molecular Weight | 17 aa, 8 kDa |
| Observed Molecular Weight | 18-23 kDa |
| GenBank Accession Number | BC015037 |
| Gene Symbol | MAFF |
| Gene ID (NCBI) | 23764 |
| RRID | AB_2137677 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9ULX9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MAFF encodes a basic region and leucine zipper (bZIP)-type transcription factor protein (PMID: 8361754). MAFF interacts with the upstream promoter region of oxytocin receptor (OXTR) and plays a role in OXTR upregulation (PubMed: 7798245, PubMed:10527846). Mice lacking MAFF, MAFG and MAFK are embryonic lethal (PMID: 22158967). MAFF has a higher expression in adipose, colon, lung, prostate and skeletal muscle tissues (PMID: 24304889).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MAFF antibody 12771-1-AP | Download protocol |
| IP protocol for MAFF antibody 12771-1-AP | Download protocol |
| WB protocol for MAFF antibody 12771-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Recognition of BACH1 quaternary structure degrons by two F-box proteins under oxidative stress | ||
Sci Transl Med PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma. | ||
J Clin Invest circular RNA cia-MAF drives self-renewal and metastasis of liver tumor-initiating cells via transcription factor MAFF. | ||
Autophagy The FACT complex facilitates expression of lysosomal and antioxidant genes through binding to TFEB and TFE3. | ||
Mol Ther Nucleic Acids miR-320a induces pancreatic β cells dysfunction in diabetes by inhibiting MafF | ||
Mol Oncol Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Mary Heather Celine (Verified Customer) (09-09-2025) | This antibody produced really clear signal through immunofluorescence staining in young and aged mouse brains. I would want to see if there is a proper control to this antibody given its importance in the aging brain.
|
FH Amy (Verified Customer) (03-18-2024) | Relatively clean bands but not very strong, U2OS lysates 25ug.
|
