MAGEA10 Polyclonal antibody

MAGEA10 Polyclonal Antibody for WB, ELISA

Cat No. 15295-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Cancer/testis antigen 1.10, CT1.10, MAGE 10 antigen, MAGE10, MAGEA10, melanoma antigen family A, 10, Melanoma associated antigen 10

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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WB analysis of A375 using 15295-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 15295-1-AP (MAGEA10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Jurkat using 15295-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 15295-1-AP (MAGEA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in

A375 cells, Jurkat cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15295-1-AP targets MAGEA10 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7540 Product name: Recombinant human MAGEA10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 39-369 aa of BC004105 Sequence: SSSTSTSSSFPSSFPSSSSSSSSSCYPLIPSTPEEVSADDETPNPPQSAQIACSSPSVVASLPLDQSDEGSSSQKEESPSTLQVLPDSESLPRSEIDEKVTDLVQFLLFKYQMKEPITKAEILESVIKNYEDHFPLLFSEASECMLLVFGIDVKEVDPTGHSFVLVTSLGLTYDGMLSDVQSMPKTGILILILSIIFIEGYCTPEEVIWEALNMMGLYDGMEHLIYGEPRKLLTQDWVQENYLEYRQVPGSDPARYEFLWGPRAHAEIRKMSLLKFLAKVNGSDPRSFPLWYEEALKDEEERAQDRIATTDDTTAMASASSSATGSFSYPE Predict reactive species
Full Name	melanoma antigen family A, 10
Calculated Molecular Weight	41 kDa
Observed Molecular Weight	62 kDa, 41 kDa
GenBank Accession Number	BC004105
Gene Symbol	MAGEA10
Gene ID (NCBI)	4109
RRID	AB_2137968
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P43363
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MAGEA10 antibody 15295-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The MAGEA10 Polyclonal antibody (Cat# 15295-1-AP) has 1 total citation. It was cited in BMC Cancer (Impact Factor 4.1) in a 2018 study by Xiao Song et al., titled "Post-transcriptional regulation of cancer/testis antigen MAGEC2 expression by TRIM28 in tumor cells." The research field is cancer biology, specifically involving post-transcriptional gene regulation and tumor-associated antigens. The antibody was used for Western blot (WB) in human samples.

Species	Application	Title
human	WB	BMC Cancer Post-transcriptional regulation of cancer/testis antigen MAGEC2 expression by TRIM28 in tumor cells. Authors - Xiao Song View Article