Tested Applications
| Positive WB detected in | HepG2 cells, A375 cells |
| Positive IP detected in | HepG2 cells |
| Positive IF/ICC detected in | HepG2 cells, A375 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12400-1-AP targets MAGEB1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3094 Product name: Recombinant human MAGEB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-347 aa of BC013772 Sequence: MPRGQKSKLRAREKRRKAREETHYLKVAHATAAEKEECPSSSPVLGDTPTSSPAAGIPQKPQGAPPTTTAAAAVSCTESDEGAKCQGEENASFSQATTSTESSVKDPVAWEAGMLMHFILRKYKMREPIMKADMLKVVDEKYKEYFTEILNGASRRLELVFGLDLKEDNPSGHTYTLVSKLNLTNDGNLSNDWDFPRNGLLMPLLGVIFLKGNSATEEEIWKFMNVLGAYDGEEHLIYGEPRKFITQDLVQEKYLKYEQVPNSDPPRYQFLWGPRAYAETTKMKVLEFLAKMNGATPRDFPSHYEEALRDEEERAQVRSSVRARRRTTATTFRARSRAPFSRSSHPM Predict reactive species |
| Full Name | melanoma antigen family B, 1 |
| Calculated Molecular Weight | 347 aa, 39 kDa |
| Observed Molecular Weight | 43-45 kDa |
| GenBank Accession Number | BC013772 |
| Gene Symbol | MAGEB1 |
| Gene ID (NCBI) | 4112 |
| RRID | AB_2877854 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P43366 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MAGEB1, also named as MAGEL1, MAGEXP, DAM10 and CT3.1, is a member of the MAGEB gene family. MAGE genes encode for tumor-rejection antigens and are expressed in tumors of different histologic types and Testis/placenta, but not in other normal tissues. The MW of MAGEB1 is about 40-45kd.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MAGEB1 antibody 12400-1-AP | Download protocol |
| IP protocol for MAGEB1 antibody 12400-1-AP | Download protocol |
| WB protocol for MAGEB1 antibody 12400-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
