MAN2A1 Polyclonal antibody

MAN2A1 Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 21132-1-AP

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

Alpha-mannosidase 2, AMan II, EC:3.2.1.114, Golgi alpha-mannosidase II, MANA2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Freight/Packing: -

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Filter: AllIHCIF/ICC

IHC staining of human stomach using 21132-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 21132-1-AP (MAN2A1 antibody) at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach using 21132-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 21132-1-AP (MAN2A1 antibody) at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse stomach using 21132-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21132-1-AP (MAN2A1 antibody) at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse stomach using 21132-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21132-1-AP (MAN2A1 antibody) at dilution of 1:500 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse stomach using 21132-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21132-1-AP (MAN2A1 antibody) at dilution of 1:500 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of NIH/3T3 using 21132-1-AP
Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using MAN2A1 antibody (21132-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols
Reviews (1)

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MSDS SDS

Tested Applications

Positive IHC detected in	human stomach tissue, mouse stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	NIH/3T3 cells

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21132-1-AP targets MAN2A1 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag15536 Product name: Recombinant human MAN2A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 585-803 aa of BC142656 Sequence: GTRPFHSLMVLEKIIGNSAFLLILKDKLTYDSYSPDTFLEMDLKQKSQDSLPQKNIIRLSAEPRYLVVYNPLEQDRISLVSVYVSSPTVQVFSASGKPVEVQVSAVWDTANTISETAYEISFRAHIPPLGLKVYKILESASSNSHLADYVLYKNKVEDSGIFTIKNMINTEEGITLENSFVLLRFDQTGLMKQMMTKEDGKHHEVNVQFSWYGTTIKRD Predict reactive species
Full Name	mannosidase, alpha, class 2A, member 1
Calculated Molecular Weight	1144 aa, 131 kDa
GenBank Accession Number	BC142656
Gene Symbol	MAN2A1
Gene ID (NCBI)	4124
RRID	AB_2878816
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q16706
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.