Tested Applications
| Positive WB detected in | L02 cells, C6 cells, human liver tissue, HepG2 cells, mouse liver tissue, rat liver tissue, Raji cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human malignant melanoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 8 publications below |
| WB | See 23 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
Product Information
14410-1-AP targets TAB2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5742 Product name: Recombinant human MAP3K7IP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 344-693 aa of BC035910 Sequence: SGPRTSSTSSSVNSQTLNRNQPTVYIAASPPNTDELMSRSQPKVYISANAATGDEQVMRNQPTLFISTNSGASAASRNMSGQVSMGPAFIHHHPPKSRAIGNNSATSPRVVVTQPNTKYTFKITVSPNKPPAVSPGVVSPTFELTNLLNHPDHYVETENIQHLTDPTLAHVDRISETRKLSMGSDDAAYTQALLVHQKARMERLQRELEIQKKKLDKLKSEVNEMENNLTRRRLKRSNSISQIPSLEEMQQLRSCNRQLQIDIDCLTKEIDLFQARGPHFNPSAIHNFYDNIGFVGPVPPKPKDQRSIIKTPKTQDTEDDEGAQWNCTACTFLNHPALIRCEQCEMPRHF Predict reactive species |
| Full Name | mitogen-activated protein kinase kinase kinase 7 interacting protein 2 |
| Calculated Molecular Weight | 76 kDa |
| Observed Molecular Weight | 77 kDa |
| GenBank Accession Number | BC035910 |
| Gene Symbol | MAP3K7IP2 |
| Gene ID (NCBI) | 23118 |
| RRID | AB_2281638 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NYJ8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Tab2 has a role in the inflammatory signal transduction pathway and is involved in the reversal of NCoR-dependent and NFkB- or APP-mediated gene repression and androgen antagonist response. Besides, it is identified as a cofactor for ERBB4 to assemble NCoR corepressor complex in response to neuregulin [PMID:16469706]. It also functions as an adaptor that links TAK1 and TRAF6 in response to interleukin-1β (IL-1β) or tumour necrosis factor-α (TNF-α), and thereby mediates TAK1 activation [PMID:11460167].
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TAB2 antibody 14410-1-AP | Download protocol |
| IHC protocol for TAB2 antibody 14410-1-AP | Download protocol |
| IP protocol for TAB2 antibody 14410-1-AP | Download protocol |
| WB protocol for TAB2 antibody 14410-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Cyclophilin J limits inflammation through the blockage of ubiquitin chain sensing. | ||
Cell Biol Toxicol microRNA-149-5p mediates the PM2.5-induced inflammatory response by targeting TAB2 via MAPK and NF-κB signaling pathways in vivo and in vitro.
| ||
Front Immunol MicroRNA 27a-3p Regulates Antimicrobial Responses of Murine Macrophages Infected byMycobacterium aviumsubspeciesparatuberculosisby Targeting Interleukin-10 and TGF-β-Activated Protein Kinase 1 Binding Protein 2. | ||
J Neuroinflammation miR-155 and miR-146a collectively regulate meningitic Escherichia coli infection-mediated neuroinflammatory responses. | ||
Front Immunol Hepatitis B Surface Antigen Suppresses the Activation of Nuclear Factor Kappa B Pathway via Interaction With the TAK1-TAB2 Complex. |
