Tested Applications
| Positive WB detected in | HK-2 cells, Jurkat cells, PC-3 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human skeletal muscle tissue, human liver tissue, human placenta tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55247-1-AP targets MAP4K4 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | mitogen-activated protein kinase kinase kinase kinase 4 |
| Calculated Molecular Weight | 151 kDa |
| Observed Molecular Weight | 130-160 kDa |
| GenBank Accession Number | NM_004834 |
| Gene Symbol | MAP4K4 |
| Gene ID (NCBI) | 9448 |
| RRID | AB_10836939 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95819 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MAP4K4, also named as RAB8IPL1, GLK and MEKKK4, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. It may play a role in the response to environmental stress. MAP4K4 appears to act upstream of the JUN N-terminal pathway. This antibody is specific to MAP4K4.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MAP4K4 antibody 55247-1-AP | Download protocol |
| IP protocol for MAP4K4 antibody 55247-1-AP | Download protocol |
| WB protocol for MAP4K4 antibody 55247-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MAP4K4 Polyclonal antibody (Cat# 55247-1-AP) has 19 citations across journals including Cancer Cell, ACS Nano, Advanced Science, International Journal of Biological Sciences, Journal of Neuroinflammation, EBioMedicine, eLife, and Analytical Chemistry. Research fields span oncology (hepatocellular, lung, breast, prostate, and cervical cancers), cardiovascular disease (myocardial injury, hypertrophy, cardiotoxicity), neurology (neuropathic pain, blood-brain barrier), metabolic disorders (diabetes, lipid metabolism), and tissue regeneration.
| Species | Application | Title |
|---|---|---|
Cancer Cell Cancer stem cells orchestrate immune evasion through extracellular vesicle-mediated non-canonical signaling pathways. | ||
ACS Nano Nanoparticle-Patch System for Localized, Effective, and Sustained miRNA Administration into Infarcted Myocardium to Alleviate Myocardial Ischemia-Reperfusion Injury | ||
Adv Sci (Weinh) Peptide Transporter 1-Mediated Dipeptide Transport Promotes Hepatocellular Carcinoma Metastasis by Activating MAP4K4/G3BP2 Signaling Axis | ||
Int J Biol Sci N6-methyladenosine Reader IGF2BP2-modified HMMR Promotes Non-small Cell Lung Cancer Metastasis via Interaction with MAP4K4 | ||
J Neuroinflammation Transcriptome profiling of microRNAs reveals potential mechanisms of manual therapy alleviating neuropathic pain through microRNA-547-3p-mediated Map4k4/NF-κb signaling pathway | ||
Phytomedicine Artesunate improves glucose and lipid metabolism in db/db mice by regulating the metabolic profile and the MAPK/PI3K/Akt signalling pathway |
