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MARCKS Polyclonal antibody

MARCKS Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 51087-1-AP

Synonyms

80K L, 80K L protein, MACS, MARCKS, PKCSL, PRKCSL

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using MARCKS Polyclonal antibody (51087-1-AP)

    WB analysis of mouse brain using 51087-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 51087-1-AP (MARCKS Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using MARCKS Polyclonal antibody (51087-1-AP)

    WB analysis of mouse brain using 51087-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 51087-1-AP (MARCKS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

51087-1-AP targets MARCKS in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name myristoylated alanine-rich protein kinase C substrate
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa, 80 kDa
GenBank Accession NumberBC089040
Gene Symbol MARCKS
Gene ID (NCBI) 4082
RRIDAB_2881243
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MARCKS(Myristoylated alanine-rich C-kinase substrate, 32 kDa ) is also named as MACS, PRKCSL and belongs to the MARCKS family. It is also named as protein kinase C substrate(80 kDa protein, light chain). MARCKS is a filamentous actincrosslinking protein that appears to function as an integrator of protein kinase C and calcium (Ca2+)/calmodulin signals in the regulation of actin-membrane interactions. This protein can be Phosphorylated by PKC displaces MARCKS from the membrane.

Protocols

Product Specific Protocols
WB protocol for MARCKS antibody 51087-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols