Tested Applications
| Positive WB detected in | COLO 320 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Caco-2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25667-1-AP targets MARVELD3 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22492 Product name: Recombinant human MARVELD3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-189 aa of BC013376 Sequence: MEDPSGAREPRARPRERDPGRRPHPDQGRTHDRPRDRPGDPRRKRSSDGNRRRDGDRDPKRDQERDGNRDRNRDRERERERERDPDRGPRRDTHRDAGPRAGEHGVWEKPRQSRTRDGARGLTWDAAAPPGPAPWEAPEPPQPQRKGDPGRRRPESEPPSERYLPSTPRPGREEVEYYQSEAEGLLECH Predict reactive species |
| Full Name | MARVEL domain containing 3 |
| Calculated Molecular Weight | 401 aa, 45 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC013376 |
| Gene Symbol | MARVELD3 |
| Gene ID (NCBI) | 91862 |
| RRID | AB_2880184 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96A59 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MARVELD3 (MARVEL domain-containing protein 3), a four-span transmembrane protein of about 40 kDa, is a tight junction-associated transmembrane protein of the occludin family (PMID: 20028514). MARVELD3 functions as a determinant of epithelial paracellular permeability properties. It has been reported that MARVELD3 couples tight junctions to the MEKK1-JNK pathway to regulate cell behavior and survival (PMID: 24567356).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MARVELD3 antibody 25667-1-AP | Download protocol |
| IHC protocol for MARVELD3 antibody 25667-1-AP | Download protocol |
| WB protocol for MARVELD3 antibody 25667-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
PLoS One Pinoresinol enhances oral barrier integrity and function in human buccal cell monolayers | ||
Int J Biol Macromol Molecular weight-dependent hyaluronic acid permeability and tight junction modulation in human buccal TR146 cell monolayers | ||
Mol Med Upregulation of PRRX2 by silencing Marveld3 as a protective mechanism against radiation-induced ferroptosis in skin cells | ||
FEBS Open Bio Hesperidin enhances intestinal barrier function in Caco-2 cell monolayers via AMPK-mediated tight junction-related proteins | ||
Food Chem Toxicol Polystyrene nanoplastics compromise oral epithelial barrier integrity and alter tight junction-related protein organization in TR146 human buccal epithelial cells. | ||
Cell Signal MarvelD3 regulates the radiosensitivity of Esophageal squamous cell carcinoma via the MYB/IFI6 axis.
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