Tested Applications
| Positive WB detected in | HEK-293 cells, PC-3 cells |
| Positive IP detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10426-1-AP targets MAX in WB, IHC, IF, IP, CoIP, chIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0680 Product name: Recombinant human MAX protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-151 aa of BC003525 Sequence: MSDNDDIEVESDADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKASRAQILDKATEYIQYMRRKNHTHQQDIDDLKRQNALLEQQVRALEKARSSAQLQTNYPSSDNSLYTNAKGSTISAFDGGSDSSSESEPEEPQSRKKLRMEAS Predict reactive species |
| Full Name | MYC associated factor X |
| Calculated Molecular Weight | 18 kDa |
| Observed Molecular Weight | 22 kDa |
| GenBank Accession Number | BC003525 |
| Gene Symbol | MAX |
| Gene ID (NCBI) | 4149 |
| RRID | AB_2141660 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P61244 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Max, a member of MAX family, contains a basic helix-loop-helix (bHLH) domain, is a transcription regulator. MAX can form a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC-MAX complex is a transcriptional activator, while the MAD-MAX complex is a repressor. MAX could dimerizated with another bHLH protein to form a heterodimer, such MYC or MAD. Thus bands recognized by this antibody much larger than predicted.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for MAX antibody 10426-1-AP | Download protocol |
| WB protocol for MAX antibody 10426-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MAX antibody (Cat# 10426-1-AP) has 25 total citations. These appear in journals including Cancer Cell, Cell, Nature Communications, Molecular Cell, Science Advances, Cell Reports, eLife, Journal of Biological Chemistry, and others. Research fields span oncology (MYC/MAX network, tumor suppression, metastasis), epigenetics and transcriptional regulation, stem cell biology, embryonic development, metabolism, and immunology. Applications include WB, ChIP, IF, IHC, IP, and CoIP.
| Species | Application | Title |
|---|---|---|
Cancer Cell MAX Functions as a Tumor Suppressor and Rewires Metabolism in Small Cell Lung Cancer.
| ||
Cell MYC binding to nascent RNA suppresses innate immune signaling by R-loop-derived RNA-DNA hybrids | ||
Nat Commun Counteracting FOX proteins epigenetically control the herpesvirus lytic-latent balance. | ||
Nat Commun PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression. | ||
Nat Commun Unreprogrammed H3K9me3 prevents minor zygotic genome activation and lineage commitment in SCNT embryos | ||
Mol Cell The MYCN oncoprotein is an RNA-binding accessory factor of the nuclear exosome targeting complex |
Reviews
What customers say
The single review (5/5) is from a researcher who used this antibody for ChIP, Western blot, and immunofluorescence on mouse embryonic stem cells at a 1:1000 dilution. They reported excellent performance across all applications and summarized their experience by saying "Life is much easier when you have a good antibody." Overall, the feedback is strongly positive, highlighting reliable results and ease of use.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Inmaculada (Verified Customer) (02-12-2026) | I used this antibody for ChIP, Western blot, and immunofluorescence, and it performs very well. Life is much easier when you have a good antibody.
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