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KD/KO Validated

MBD2 Polyclonal antibody

MBD2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 55200-1-AP

Publications(11)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

Demethylase, DMTase, Methyl-CpG-binding domain protein 2, Methyl-CpG-binding protein MBD2, NY CO 41

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis of Jurkat using 55200-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of A431 using 55200-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of A431 using 55200-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of A431 using 55200-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HEK-293 using 55200-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human brain using 55200-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human brain using 55200-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human brain using 55200-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human cervical cancer using 55200-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 55200-1-AP (MBD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human cervical cancer using 55200-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 55200-1-AP (MBD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (11)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells, human brain tissue, A431 cells, HEK-293 cells
Positive IHC detected in	human cervical cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55200-1-AP targets MBD2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	methyl-CpG binding domain protein 2
Calculated Molecular Weight	43 kDa
Observed Molecular Weight	43-45 kDa
GenBank Accession Number	NM_003927
Gene Symbol	MBD2
Gene ID (NCBI)	8932
RRID	AB_10852963
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UBB5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MBD2, also named as DMTase, binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. MBD2 binds hemi-methylated DNA as well. It recruits histone deacetylases and DNA methyltransferases. MBD2 acts as transcriptional repressor and plays a role in gene silencing (PMID:9774669). MBD2 belongs to a highly conserved member of the MBD protein family, is considered a reader of DNA methylation and drives the crosstalk between DNA methylation and histone deacetylation (PMID: 40089655). MBD2 drives breast cancer progression through the modulation of epithelial-to-mesenchymal transition (PMID: 38556549).

Protocols

Product Specific Protocols
IHC protocol for MBD2 antibody 55200-1-AP	Download protocol
WB protocol for MBD2 antibody 55200-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	WB	Nat Commun Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk. Authors - Xiao-Hui Wang KD Validated View Article
mouse	WB	Exp Cell Res Loss of MBD2 affects early T cell development by inhibiting the WNT signaling pathway. Authors - Ling Cheng KO Validated View Article
human	WB	Cell Rep TRPS1 drives heterochromatic origin refiring and cancer genome evolution. Authors - Jianguo Yang View Article
human	WB	Genet Test Mol Biomarkers The Expression of MBD6 Is Associated with Tumor Size in Uterine Leiomyomas. Authors - Mei-Rong Liang View Article
human	WB,IF	Gene Exploring the importance of m5c in the diagnosis and subtype classification of COPD using the GEO database Authors - Jianjun Wu View Article
	WB	Cell Immunol Aged mice-derived bronchial epithelial cells regulate Th17 cell differentiation in asthma via the MBD2-sICOSL axis Authors - Zhifeng Chen View Article