MCL1 Antibody
Mouse Monoclonal
Catalog number: 66026-1-Ig
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Applications
| Tested applications | ELISA, WB, IF |
| Cited applications | |
| Species specificity | human; other species not tested. |
| Cited species | |
| Positive WB detected in | Hela cells |
| Positive IF detected in | Hela cells |
| Recommended dilution | WB: 1:500-1:5000 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
Hela cells were subjected to SDS PAGE followed by western blot with 66026-1-Ig(MCL1 Antibody) at dilution of 1:1000
Immunofluorescent analysis of Hela cells, using MCL1 antibody 66026-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
Product Information
| Source | Mouse | Purification method | Protein A purification |
| Isotype | IgG2b | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | MCL1 fusion protein ag10609 | Full name | myeloid cell leukemia sequence 1 (BCL2-related) |
|
Calculated molecular weight |
350aa,37kd |
Observed molecular
weight |
40kD |
|
GenBank accession number |
BC107735 | Gene ID (NCBI) | 4170 |
| Gene symbol | MCL1 | Synonyms | BCL2L3; EAT; MCL1L; MCL1S; MGC104264; MGC1839; Mcl-1; TM |
Background
MCL-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Mcl-1 is critical for the proliferation and survival of myeloma cells in vitro, and overexpression of Mcl-1 protein in myeloma cells is associated with relapse and short event-free survival in multiple myeloma patients. Recent studies show that MCL-1 is upregulated in numerous haematological and solid tumour malignancies. Therefore, MCL-1 has been suggested as a potential new therapeutic target. |
