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Tested Applications
| Positive WB detected in | rat testis tissue, human testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66472-1-Ig targets MEA1 in WB, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0375 Product name: Recombinant human MEA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-185 aa of BC001754 Sequence: MGPERHLSGAPARMATVVLGGDTMGPERIFPNQTEELGHQGPSEGTGDWSSEEPEEEQEETGSGPAGYSYQPLNQDPEQEEVELAPVGDGDVVADIQDRIQALGLHLPDPPLESEDEDEEGATALNNHSSIPMDPEHVELVKRTMAGVSLPAPGVPAWAREISDAQWEDVVQKALQARQASPAWK Predict reactive species |
| Full Name | male-enhanced antigen 1 |
| Calculated Molecular Weight | 20 kDa |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC001754 |
| Gene Symbol | MEA1 |
| Gene ID (NCBI) | 4201 |
| RRID | AB_2881838 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q16626 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MEA1, also named as Male-enhanced antigen 1, is a 185 amino acid protein, which is highly expressed in testis. MEA1 may play an important role in spermatogenesis and/or testis development.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MEA1 antibody 66472-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
