MESDC2 Polyclonal antibody

MESDC2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10958-1-AP

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

BOCA, KIAA0081, LDLR chaperone MESD, LRP chaperone MESD, MESD

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

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Filter: AllWBIHC

WB analysis using 10958-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10958-1-AP (MESDC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 10958-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10958-1-AP (MESDC2 antibody) at dilution of 1:800 incubated at 4 degree celsius over night.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HEK-293 using 10958-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10958-1-AP (MESDC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 10958-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10958-1-AP (MESDC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 10958-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10958-1-AP (MESDC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 10958-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10958-1-AP (MESDC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach using 10958-1-AP
Immunohistochemical analysis of paraffin-embedded normal stomach slide using 10958-1-AP (MESDC2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach using 10958-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10958-1-AP (MESDC2 antibody) at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach using 10958-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10958-1-AP (MESDC2 antibody) at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols
Reviews (1)

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MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, NIH/3T3 cells, JAR cells
Positive IHC detected in	human placenta tissue, human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10958-1-AP targets MESDC2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1403 Product name: Recombinant human MESDC2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-234 aa of BC009210 Sequence: MAASRWARKAVVLLCASDLLLLLLLLPPPGSCAAEGSPGTPDESTPPPRKKKKDIRDYNDADMARLLEQWEKDDDIEEGDLPEHKRPSAPVDFSKIDPSKPESILKMTKKGKTLMMFVTVSGSPTEKETEEITSLWQGSLFNANYDVQRFIVGSDRAIFMLRDGSYAWEIKDFLVGQDRCADVTLEGQVYPGKGGGSKEKNKTKQDKGKKKKEGDLKSRSSKEENRAGNKREDL Predict reactive species
Full Name	mesoderm development candidate 2
Calculated Molecular Weight	26 kDa
Observed Molecular Weight	26-28 kDa
GenBank Accession Number	BC009210
Gene Symbol	MESDC2
Gene ID (NCBI)	23184
RRID	AB_2143722
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14696
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.