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KD/KO Validated

METTL9 Polyclonal antibody

METTL9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15120-1-AP

Publications(3)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CGI 81, CGI81, CGI-81, DREV, DREV1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

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Filter: AllWBIHCIF/ICC

WB analysis using 15120-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15120-1-AP (METTL9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 15120-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15120-1-AP (METTL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 15120-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15120-1-AP (METTL9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 15120-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15120-1-AP (METTL9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 15120-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15120-1-AP (METTL9 Antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 15120-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15120-1-AP (METTL9 Antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 15120-1-AP
Immunofluorescent analysis of HepG2 cells using 15120-1-AP (METTL9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (3)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15120-1-AP targets METTL9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7191 Product name: Recombinant human METTL9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 36-318 aa of BC000195 Sequence: MTSGPGGPAAAAGGRKENHQWYVCNREKLCESLQAVFVQSYLDQGTQIFLNNSIEKSGWLFIQLYHSFVSSVFSLFMSRTSINGLLGRGSMFVFSPDQFQRLLKINPDWKTHRLLDLGAGDGEVTKIMSPHFEEIYATELSETMIWQLQKKKYRVLGINEWQNTGFQYDVISCLNLLDRCDQPLTLLKDIRSVLEPTRGRVILALVLPFHPYVENVGGKWEKPSEILEIKGQNWEEQVNSLPEVFRKAGFVIEAFTRLPYLCEGDMYNDYYVLDDAVFVLKPV Predict reactive species
Full Name	methyltransferase like 9
Calculated Molecular Weight	37 kDa
Observed Molecular Weight	40 kDa
GenBank Accession Number	BC000195
Gene Symbol	METTL9
Gene ID (NCBI)	51108
RRID	AB_2878109
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H1A3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

METTL9 is a broad-specificity methyltransferase that mediates the formation of the majority of 1-methylhistidine(1MH) present in mouse and human proteomes.

Protocols

Product Specific Protocols
IF protocol for METTL9 antibody 15120-1-AP	Download protocol
IHC protocol for METTL9 antibody 15120-1-AP	Download protocol
WB protocol for METTL9 antibody 15120-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	WB	Biochem Biophys Rep Elevated METTL9 is associated with peritoneal dissemination in human scirrhous gastric cancers. Authors - Toshifumi Hara KD Validated View Article
human	WB,IHC	Cell Death Discov METTL9-SLC7A11 axis promotes hepatocellular carcinoma progression through ferroptosis inhibition Authors - Fangfang Bi View Article
human	WB,IP	Mol Med METTL9 mediated N1-Histidine methylation of SLC39A7 confers ferroptosis resistance and inhibits adipogenic differentiation in mesenchymal stem cells Authors - Jiahao Jin View Article