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MFI2 Polyclonal antibody

MFI2 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 10428-1-AP

Synonyms

Melanotransferrin, MFI2, Mtf, p97

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using MFI2 Polyclonal antibody (10428-1-AP)

    WB analysis of HEK-293 using 10428-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10428-1-AP (MFI2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MFI2 Polyclonal antibody (10428-1-AP)

    WB analysis of HeLa using 10428-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10428-1-AP (MFI2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Transfected HEK-293 cells using MFI2 Polyclonal antibody (10428-1-AP)

    WB analysis of Transfected HEK-293 using 10428-1-AP

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 10428-1-AP (MFI2 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Transfected

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10428-1-AP targets MFI2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MFI2 fusion protein Ag0700
Full Name antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5
Calculated Molecular Weight 80 kDa
Observed Molecular Weight 82 kDa
GenBank Accession NumberBC001875
Gene Symbol MFI2
Gene ID (NCBI) 4241
RRIDAB_2142592
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MFI2 (Melanotransferrin/Mtf/TRMF), also known as p97 or CD228, is an iron-binding glycoprotein belonging to the transferrin family. Alternative RNA splicing yields two forms of different length transcripts, one (the longer) bound to the membrane (mMFI2) and the other (the shorter) secreted to the peripheral environment (sMFI2) such as blood and cell supernatant. MFI2 is upregulated in tumor tissues and has been identified as a serological Marker of colorectal cancer (PMID: 25216327).

Protocols

Product Specific Protocols
WB protocol for MFI2 antibody 10428-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Death Discov

Gsα deficiency facilitates cardiac remodeling via CREB/ Bmp10-mediated signaling

Authors - Ping Yin
View Article
human,ratWB,IF

Reprod Sci

MELTF Might Regulate Ferroptosis, Pyroptosis, and Autophagy in Platelet-Rich Plasma-Mediated Endometrial Epithelium Regeneration

Authors - Yanhong Mao
View Article
humanWB

Front Immunol

Identification of novel gene signature for lung adenocarcinoma by machine learning to predict immunotherapy and prognosis

Authors - Jianfeng Shu
View Article