Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
| Positive IP detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
17841-1-AP targets MGAT4C in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12201 Product name: Recombinant human MGAT4C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 127-478 aa of BC064141 Sequence: SSYEELKEISVVVHLADFNSSWRDAMVQDITQKFAHHIIAGRLMVIHAPEEYYPILDGLKRNYNDPEDRVKFRSKQNVDYAFLLNFCANTSDYYVMLEDDVRCSKNFLTAIKKVIASLEGTYWVTLEFSKLGYIGKLYHSHDLPRLAHFLLMFYQEMPCDWLLTHFRGLLAQKNVIRFKPSLFQHMGYYSSYKGTENKLKDDDFEEESFDIPDNPPASLYTNMNVFENYEASKAYSSVDEYFWGKPPSTGDVFVIVFENPIIIKKIKVNTGTEDRQNDILHHGALDVGENVMPSKQRRQCSSYLRLGEFKNGNFEMSGVNQKIPFDIHCMRIYVTKTQKEWLIIRSISIWTS Predict reactive species |
| Full Name | mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme C (putative) |
| Calculated Molecular Weight | 478 aa, 56 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC064141 |
| Gene Symbol | MGAT4C |
| Gene ID (NCBI) | 25834 |
| RRID | AB_2143048 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBM8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MGAT4C is predicted to be a glycosyltransferase that catalyzes a GlcNAc β1-4 linkage to the core mannose residues of N-glycans, resulting in N-glycans with tri- or tetraantennary structures.Recently MGAT4C has been found to get involved in processing complex N-glycan structures on CD133 and CD133 has been identified as a substrate of MGAT4C. Given to the fact that CD133 is widely used as a marker of numerous stem cell and cancer stem cell types, MGAT4C may have an important role in certain cancers. (PMID: 21937449)
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for MGAT4C antibody 17841-1-AP | Download protocol |
| WB protocol for MGAT4C antibody 17841-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Dev Cell Wound-healing plasticity enables clonal expansion of founder progenitor cells in colitis | ||
Clin Transl Med Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1. | ||
J Biol Chem CD133 protein N-glycosylation processing contributes to cell surface recognition of the primitive cell marker AC133 epitope.
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