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WB analysis of HepG2 using 16993-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 16993-1-AP (MGAT5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 16993-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16993-1-AP (MGAT5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 16993-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 16993-1-AP (MGAT5B antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 16993-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 16993-1-AP (MGAT5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of C6 using 16993-1-AP
C6 cells were subjected to SDS PAGE followed by western blot with 16993-1-AP (MGAT5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 16993-1-AP
IP result of anti-MGAT5B (IP:16993-1-AP, 4ug; Detection:16993-1-AP 1:700) with Jurkat cells lysate 3600ug.
IHC staining of mouse brain using 16993-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16993-1-AP (MGAT5B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 16993-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16993-1-AP (MGAT5B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 16993-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16993-1-AP (MGAT5B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 16993-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16993-1-AP (MGAT5B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 16993-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16993-1-AP (MGAT5B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

MGAT5B Polyclonal antibody

Name: MGAT5B Polyclonal antibody
Brand: Proteintech
SKU: 16993-1-AP

MGAT5B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16993-1-AP

Publications(3)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

GlcNAc T Vb, GnT IX, GnT VB, hGnTVb, KIAA2008, MGAT5B

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HepG2 cells, Jurkat cells, SH-SY5Y cells, MCF-7 cells
Positive IP detected in	Jurkat cells
Positive IHC detected in	mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

16993-1-AP targets MGAT5B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity	human, rat, mouse
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10678 Product name: Recombinant human MGAT5B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-394 aa of BC062354 Sequence: MITVNPDGKIMVRRCLVTLRPFRLFVLGIGFFTLCFLMTSLGGQFSARRLGDSPFTIRTEVMGGPESRGVLRKMSDLLELMVKRMDALARLENSSELHRAGGDLHFPADRMPPGAGLMERIQAIAQNVSDIAVKVDQILRHSLLLHSKVSEGRRDQCEAPSDPKFPDCSGKVEWMRARWTSDPCYAFFGVDGTECSFLIYLSEVEWFCPPLPWRNQTAAQRAPKPLPKVQAVFRSNLSHLLDLMGSGKESLIFMKKRTKRLTAQWALAAQRLAQKLGATQRDQKQILVHIGFLTEESGDVFSPRVLKGGPLGEMVQWADILTALYVLGHGLRVTVSLKELQRQRRLRSSQEQARCGVMGACEKMLLEGDVASNTLLIEKPSKKETEKCPKNLVT Predict reactive species
Full Name	mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, isozyme B
Calculated Molecular Weight	394 aa, 44 kDa, 89 kDa
Observed Molecular Weight	89-100 kDa
GenBank Accession Number	BC062354
Gene Symbol	MGAT5B
Gene ID (NCBI)	146664
RRID	AB_10858919
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q3V5L5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for MGAT5B antibody 16993-1-AP	Download protocol
IHC protocol for MGAT5B antibody 16993-1-AP	Download protocol
IP protocol for MGAT5B antibody 16993-1-AP	Download protocol
WB protocol for MGAT5B antibody 16993-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human, rat	WB	Schizophr Res Abnormal N-acetylglucosaminyltransferase expression in prefrontal cortex in schizophrenia. Authors - Jordyn M Kippe View Article
human	IHC	Medicine (Baltimore) Pathological discrimination between luteinized thecoma associated with sclerosing peritonitis and thecoma Authors - Jia Liu View Article
human	WB,IF	J Biol Chem Brain-specific glycosylation enzyme GnT-IX maintains levels of protein tyrosine phosphatase receptor PTPRZ, thereby mediating glioma growth Authors - Kenichiro Nagai View Article