MKP-2 Monoclonal antibody

MKP-2 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat, mouse

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1G1C12

Cat no : 66349-1-Ig

Synonyms

dual specificity phosphatase 4, DUSP4, HVH2, MAP kinase phosphatase 2, MKP 2, MKP2, MKP-2, TYP, VH2



Tested Applications

Positive WB detected inpig brain tissue, RAW 264.7 cells, HepG2 cells, PC-12 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66349-1-Ig targets MKP-2 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, pig, rat, mouse samples.

Tested Reactivity human, pig, rat, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MKP-2 fusion protein Ag1206
Full Name dual specificity phosphatase 4
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa, 33 kDa
GenBank Accession NumberBC014565
Gene Symbol DUSP4
Gene ID (NCBI) 1846
RRIDAB_2881729
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DUSP4, also named as MKP-2, is a member of the mitogen-activated protein (MAP) kinase phosphatase family which has been suggested to play an important role in the feedback control of MAP kinase-mediated gene expression. DUSP4 preferentially inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. DUSP4 encodes two distinct isoforms: MKP-2-L(43 kDa) and MKP-2-S (33 kDa).

Protocols

Product Specific Protocols
WB protocol for MKP-2 antibody 66349-1-IgDownload protocol
IHC protocol for MKP-2 antibody 66349-1-IgDownload protocol
IF protocol for MKP-2 antibody 66349-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

Mol Neurobiol

Dual Specificity Phosphatases Support Axon Plasticity and Viability.

Authors - Ambika Chandrasekhar
humanWB,IHC

J Cancer

PMID: 31258782

Authors - Xianqing Chen
humanWB

Cell Death Dis

Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal degeneration

Authors - Xiaoman Zhu
human,mouseWB,IHC,CoIP

Cell Rep

DUSP4 promotes esophageal squamous cell carcinoma progression by dephosphorylating HSP90β

Authors - Liting Zhou
  • KD Validated
humanIHC

Cancers (Basel)

Primary Colorectal Tumor Displays Differential Genomic Expression Profiles Associated with Hepatic and Peritoneal Metastases

Authors - Maximiliano Gelli
humanIF

Life Sci Alliance

DOCK1 insufficiency disrupts trophoblast function and pregnancy outcomes via DUSP4-ERK pathway

Authors - Yichi Xu