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Tested Applications
| Positive WB detected in | mouse kidney tissue, A431 cells, human brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
55102-1-AP targets AF-6 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4 |
| Calculated Molecular Weight | 207 kDa |
| Observed Molecular Weight | 182-210 kDa |
| GenBank Accession Number | NM_005936 |
| Gene Symbol | Afadin/AF6 |
| Gene ID (NCBI) | 4301 |
| RRID | AB_10792809 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P55196 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MLLT4, also named as AF6 and Afadin, belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). It is a nNectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton. The antibody is specific to MLLT4.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AF-6 antibody 55102-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Ethnopharmacol Wuzi-Yanzong-Wan prevents oligoasthenospermia due to TAp73 suppression by affecting cellular junction remodeling in testicular tissue in mice | ||
Res Sq Novel Populations of Lung Capillary Endothelial Cells and Their Functional Significance | ||
Aging (Albany NY) The mechanism of simultaneous intake of Jujuboside A and B in the regulation of sleep at the hypothalamic level |
