MLPH Antibody
Rabbit Polyclonal
Catalog number: 10338-1-AP
Ask a question about this product click here
Loading price...
Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | FC, IHC, WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human |
| Positive WB detected in | A375 cells |
| Positive IHC detected in | human skin cancer |
| Positive IF detected in | MCF-7 cells |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:20-1:200 IF: 1:20-1:100 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
A375 cells were subjected to SDS PAGE followed by western blot with 10338-1-AP(MLPH Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human skin cancer using 10338-1-AP(MLPH Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human skin cancer using 10338-1-AP(MLPH Antibody) at Dilution 1:50 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells, using MLPH antibody 10338-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | MLPH fusion protein ag0316 | Full name | melanophilin |
|
Calculated molecular weight |
66kd |
Observed molecular
weight |
66kd,80kd |
|
GenBank accession number |
BC001653 | Gene ID (NCBI) | 79083 |
| Gene symbol | MLPH | Synonyms | MGC2771; MGC59733; SLAC2-A |
Background
Melanophilin (Mlph) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft. |
