MMP13 Antibody
Rabbit Polyclonal
Catalog number: 18165-1-AP
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Applications
| Tested applications | ELISA, WB, IHC |
| Cited applications | IHC, WB |
| Species specificity | human, mouse, rat, zebrafish; other species not tested. |
| Cited species | human, mouse, rabbit |
| Positive WB detected in | A2780 cells |
| Positive IHC detected in | human breast cancer |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:20-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
A2780 cells were subjected to SDS PAGE followed by western blot with 18165-1-AP(MMP13 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human breast cancer using 18165-1-AP(MMP13 Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human breast cancer using 18165-1-AP(MMP13 Antibody) at Dilution 1:50 (under 40x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | MMP13 fusion protein ag12653 | Full name | matrix metallopeptidase 13 (collagenase 3) |
|
Calculated molecular weight |
471aa,54kd |
Observed molecular
weight |
50-52kd |
|
GenBank accession number |
BC074808 | Gene ID (NCBI) | 4322 |
| Gene symbol | MMP13 | Synonyms | CLG3 |
Background
MMP-13(Matrix metalloproteinase-13), also known as collagenase-3, cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial collagenase (MMP-1). MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate.The bands seen with rat MMP-13 are consistent with a 59-kDa latent form and a 43-kDa active form reported for this enzyme ,along with a smaller band at approximately 30 kDa(PMID:10771523).This antibody is specific to MMP13. |
