Filter: AllWBIHCIF/ICC

WB analysis using 14416-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14416-1-AP (MOCS2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 14416-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14416-1-AP (MOCS2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14416-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14416-1-AP (MOCS2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 14416-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14416-1-AP (MOCS2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of rat heart using 14416-1-AP
Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 14416-1-AP (MOCS2B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat heart using 14416-1-AP
Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 14416-1-AP (MOCS2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 14416-1-AP
Immunofluorescent analysis of HeLa cells using 14416-1-AP (MOCS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

MOCS2B Polyclonal antibody

MOCS2B Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 14416-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

MOCS2, MOCO1-B, MOCO1-A, MOCO1 A, MOCO1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, Jurkat cells, mouse heart tissue
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14416-1-AP targets MOCS2B in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5761 Product name: Recombinant human MOCS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-188 aa of BC046097 Sequence: MSSLEISSSCFSLETKLPLSPPLVEDSAFEPSRKDMDEVEEKSKDVINFTAEKLSVDEVSQLVISPLCGAISLFVGTTRNNFEGKKVISLEYEAYLPMAENEVRKICSDIRQKWPVKHIAVFHRLGLVPVSEASIIIAVSSAHRAASLEAVSYAIDTLKAKVPIWKKEIYEESSTWKGNKECFWASNS Predict reactive species
Full Name	molybdenum cofactor synthesis 2
Calculated Molecular Weight	21 kDa
Observed Molecular Weight	21 kDa
GenBank Accession Number	BC046097
Gene Symbol	MOCS2
Gene ID (NCBI)	4338
RRID	AB_2250779
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O96033
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.