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WB analysis of MCF-7 using 10695-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10695-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of MDA-MB-453s using 10695-1-AP
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10695-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10695-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10695-1-AP
IP result of anti-MPHOSPH6 (IP:10695-1-AP, 4ug; Detection:10695-1-AP 1:500) with HeLa cells lysate 3000 ug.
IHC staining of human breast cancer using 10695-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 10695-1-AP (MPHOSPH6 antibody) at dilution of 1:50 (under 10x lens).

MPHOSPH6 Polyclonal antibody

Name: MPHOSPH6 Polyclonal antibody
Brand: Proteintech
SKU: 10695-1-AP
Rating: 5.00 (2 reviews)

Cat No. 10695-1-AP

Publications(1)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Synonyms

M phase phosphoprotein 6, MPHOSPH6, MPP, MPP 6, MPP6

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

Freight/Packing: -

Quantity

- +

Positive WB detected in	MCF7 cells, HeLa cells, MCF-7 cells, MDA-MB-453s cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

10695-1-AP targets MPHOSPH6 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1080 Product name: Recombinant human MPHOSPH6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-160 aa of BC005242 Sequence: MAAERKTKLSKNLLRMKFMQRGLDSETKKQLEEEEKKIISEEHWYLDLPELKEKESFIIEEQSFLLCEDLLYGRMSFRGFNPEVEKLMLQMNAKHKAEEVEDETVELDVSDEEMARRYETLVGTIGKKFARKRDHANYEEDENGDITPIKAKKMFLKPQD Predict reactive species
Full Name	M-phase phosphoprotein 6
Calculated Molecular Weight	19 kDa
Observed Molecular Weight	19 kDa
GenBank Accession Number	BC005242
Gene Symbol	MPHOSPH6
Gene ID (NCBI)	10200
RRID	AB_2282161
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q99547
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IHC protocol for MPHOSPH6 antibody 10695-1-AP	Download protocol
IP protocol for MPHOSPH6 antibody 10695-1-AP	Download protocol
WB protocol for MPHOSPH6 antibody 10695-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
		Cell Prolif The 5.8S pre-rRNA maturation factor, M-phase phosphoprotein 6, is a female fertility factor required for oocyte quality and meiosis. Authors - Rui-Rui Peng View Article

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH Tsimafei (Verified Customer) (12-17-2023)	Nice antibody that specifically recognise Mphosph6 Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: mESCs MPHOSPH6 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:10695-1-AP)
FH Ralf (Verified Customer) (08-04-2022)	10µg of the total mouse protein were loaded on the gel. Antibodies also stain the ladder a bit. Applications: Western Blot Primary Antibody Dilution: 1:1000 MPHOSPH6 Antibody Western Blot validation (1:1000 dilution) in (Cat no:10695-1-AP)