MRPS24 Polyclonal antibody

MRPS24 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16213-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

bMRP 47, bMRP47, bMRP-47, HSPC335, MRP S24

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIHC

WB analysis using 16213-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16213-1-AP (MRPS24 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach using 16213-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16213-1-AP (MRPS24 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in	HeLa cells, HepG2 cells, L02 cells
Positive IHC detected in	human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

16213-1-AP targets MRPS24 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9211 Product name: Recombinant human MRPS24 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-167 aa of BC012167 Sequence: MAASVCSGLLGPRVLSWSRELPCAWRALHTSPVCAKNRAARVRVSKGDKPVTYEEAHAPHYIAHRKGWLSLHTGNLDGEDHAAERTVEDVFLRKFMWGTFPGCLADQLVLKRRGNQLEICAVVLRQLSPHKYYFLVGYSETLLSYFYKCPVRLHLQTVPSKVVYKYL Predict reactive species
Full Name	mitochondrial ribosomal protein S24
Calculated Molecular Weight	167 aa, 19 kDa
Observed Molecular Weight	15-19 kDa
GenBank Accession Number	BC012167
Gene Symbol	MRPS24
Gene ID (NCBI)	64951
RRID	AB_2180356
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96EL2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IHC protocol for MRPS24 antibody 16213-1-AP	Download protocol
WB protocol for MRPS24 antibody 16213-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
mouse	WB	Methods Mol Biol Linear Density Sucrose Gradients to Study Mitoribosomal Biogenesis in Tissue-Specific Knockout Mice. Authors - Benedetta Ruzzenente View Article
mouse	WB	Cell Death Discov Circular RNA circTRPS1-2 inhibits the proliferation and migration of esophageal squamous cell carcinoma by reducing the production of ribosomes Authors - Renchang Zhao View Article