MRPS27 Antibody
Rabbit Polyclonal
Catalog number: 17280-1-AP
Ask a question about this product click here
Loading price...
Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human |
| Positive WB detected in | HeLa cells, A431 cells, human heart tissue, human skeletal muscle tissue |
| Positive IHC detected in | human kidney |
| Positive IF detected in | HepG2 cells |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:50-1:200 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
HeLa cells were subjected to SDS PAGE followed by western blot with 17280-1-AP(MRPS27 Antibody) at dilution of 1:1500
Immunohistochemistry of paraffin-embedded human kidney using 17280-1-AP(MRPS27 Antibody) at Dilution 1:100 (under 10x lens)
Immunohistochemistry of paraffin-embedded human kidney using 17280-1-AP(MRPS27 Antibody) at Dilution 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using MRPS27 antibody 17280-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | MRPS27 fusion protein ag11047 | Full name | mitochondrial ribosomal protein S27 |
|
Calculated molecular weight |
168aa,19kd,48KD |
Observed molecular
weight |
48kd |
|
GenBank accession number |
BC030521 | Gene ID (NCBI) | 23107 |
| Gene symbol | MRPS27 | Synonyms | FLJ21764; FLJ23348; KIAA0264; MRP-S27; S27mt |
Background
Genes encoding PPR (pentatricopeptide repeat)-containing proteins constitute one of the largest gene families in plants. The majority of these proteins are predicted to target organelles and to bind to RNA. Strikingly, there is a dearth of these proteins in mammals, although genomic searches reveal six candidates, all of which are also predicted to target the mitochondrion. One of these proteins, MRPS27is participated in transcription and translation respectively. [PMID:18939947] MRPS27is a component of the small subunit of the mitochondrial ribosome that is encoded by the nuclear genome [PMID: 11279123]. |
