Tested Applications
| Positive WB detected in | Raji cells, Daudi cells, Ramos cells |
| Positive IHC detected in | human tonsillitis tissue, human appendicitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
| Positive IF/ICC detected in | Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:15000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60271-1-Ig targets CD20 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17309 Product name: Recombinant human MS4A1,CD20 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 141-297 aa of BC002807 Sequence: IKISHFLKMESLNFIRAHTPYINIYNCEPANPSEKNSPSTQYCYSIQSLFLGILSVMLIFAFFQELVIAGIVENEWKRTCSRPKSNIVLLSAEEKKEQTIEIKEEVVGLTETSSQPKNEEDIEIIPIQEEEEEETETNFPEPPQDQESSPIENDSSP Predict reactive species |
| Full Name | membrane-spanning 4-domains, subfamily A, member 1 |
| Calculated Molecular Weight | 297 aa, 33 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC002807 |
| Gene Symbol | CD20 |
| Gene ID (NCBI) | 931 |
| RRID | AB_2881391 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P11836 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD20 antibody 60271-1-Ig | Download protocol |
| IHC protocol for CD20 antibody 60271-1-Ig | Download protocol |
| WB protocol for CD20 antibody 60271-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Sci Transl Med The m6A demethylase FTO links TLR7 to mitochondrial oxidation driving age-associated B cell formation in systemic lupus erythematosus | ||
Clin Transl Med Single-cell transcriptome sequencing of B-cell heterogeneity and tertiary lymphoid structure predicts breast cancer prognosis and neoadjuvant therapy efficacy | ||
Cancer Lett Single-cell profiling identifies biomarkers for immunochemotherapy in esophageal squamous cell carcinoma | ||
Chin Med J (Engl) Plasma exchange and intravenous immunoglobulin prolonged the survival of a porcine kidney xenograft in a sensitized, deceased human recipient | ||
FASEB J Tertiary lymphoid structures: Associated multiple immune cells and analysis their formation in hepatocellular carcinoma | ||
Int Immunopharmacol Peritumoral TIGIT+CD20+ B cell infiltration indicates poor prognosis but favorable adjuvant chemotherapeutic response in gastric cancer. |
