Tested Applications
| Positive WB detected in | BV-2 cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11864-1-AP targets MS4A7 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2442 Product name: Recombinant human MS4A7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-240 aa of BC020673 Sequence: MLLQSQTMGVSHSFTPKGITIPQREKPGHMYQNEDYLQNGLPTETTVLGTVQILCCLLISSLGAILVFAPYPSHFNPAISTTLMSGYPFLGALCFGITGSLSIISGKQSTKPFDLSSLTSNAVSSVTAGAGLFLLADSMVALRTASQHCGSEMDYLSSLPYSEYYYPIYEIKDCLLTSVSLTGVLVVMLIFTVLELLLAAYSSVFWWKQLYSNNPGSSFSSTQSQDHIQQVKKSSSRSWI Predict reactive species |
| Full Name | membrane-spanning 4-domains, subfamily A, member 7 |
| Calculated Molecular Weight | 240 aa, 26 kDa |
| Observed Molecular Weight | 26 kDa |
| GenBank Accession Number | BC020673 |
| Gene Symbol | MS4A7 |
| Gene ID (NCBI) | 58475 |
| RRID | AB_2144674 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9GZW8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The human MS4A family currently includes 18 members, and these proteins have four putative transmembrane domains (tetraspan) and several protein kinase C (PKC) phosphorylation sites. Membrane-spanning 4-domains a7 (MS4A7) is a member of the MS4A family. It is expressed by myeloid cells. MS4A7 can act as a NAM-specific pathogenic factor that exacerbates metabolic dysfunction-associated steatohepatitis (MASH) progression in mice (PMID: 38478630). There are two isoforms of MS4A7 protein, with molecular weights of 26.14 kDa and 21.41 kDa respectively.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MS4A7 antibody 11864-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
