Tested Applications
| Positive WB detected in | MCF-7 cells, A375 cells, HeLa cells, DU 145 cells, HL-60 cells, A549 cells, mouse spleen tissue, PC-3 cells, HepG2 cells, RAW 264.7 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue, mouse brain tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13860-1-AP targets AEG-1/MTDH in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4840 Product name: Recombinant human AEG-1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 233-582 aa of BC045642 Sequence: EQLTTASFPVGSKKNKGDSHLNVQVSNFKSGKGDSTLQVSSGLNENLTVNGGGWNEKSVKLSSQISAGEEKWNSVSPASAGKRKAEPSAWSQDTGDANTNGKDWGRSWSDRSIFSGIGSTAEPVSQSTTSDYQWDVSRNQPYIDDEWSGLNGLSSADPNSDWNAPAEEWGNWVDEERASLLKSQEPIPDDQKVSDDDKEKGEGALPTGKSKKKKKKKKKQGEDNSTAQDTEELEKEIREDLPVNTSKTRPKQEKAFSLKTISTSDPAEVLVKNSQPIKTLPPATSTEPSVILSKSDSDKSSSQVPPILQETDKSKSNTKQNSVPPSQTKSETSWESPKQIKKKKKARRET Predict reactive species |
| Full Name | metadherin |
| Calculated Molecular Weight | 582 aa, 64 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC045642 |
| Gene Symbol | AEG-1/MTDH |
| Gene ID (NCBI) | 92140 |
| RRID | AB_2266734 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86UE4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression. This antibody got two bands about 70 kDa and 80 kDa in western blotting, possibly due to posttranslational modification.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AEG-1/MTDH antibody 13860-1-AP | Download protocol |
| IHC protocol for AEG-1/MTDH antibody 13860-1-AP | Download protocol |
| IP protocol for AEG-1/MTDH antibody 13860-1-AP | Download protocol |
| WB protocol for AEG-1/MTDH antibody 13860-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MTDH (AEG-1/Metadherin) Rabbit Monoclonal Antibody (Cat# 13860-1-AP) has accumulated 64 citations published across over 49 distinct journals, including high-impact outlets such as Nature Cancer, Nature Communications, PNAS, Cancer Letters, Oncogene, Journal of Biological Chemistry, Molecular Therapy, and Scientific Reports. Associated research fields broadly encompass oncology (hepatocellular carcinoma, glioma, bladder, breast, gastric, cervical, pancreatic, thyroid, and head & neck cancers), neurology, inflammatory diseases, ferroptosis, epithelial-mesenchymal transition, angiogenesis, chemoresistance, vascular biology, and microRNA-mediated gene regulatory mechanisms.
| Species | Application | Title |
|---|---|---|
Nat Commun Characterization of dsRNA binding proteins through solubility analysis identifies ZNF385A as a dsRNA homeostasis regulator | ||
Cancer Lett Metadherin regulates metastasis of squamous cell carcinoma of the head and neck via AKT signalling pathway-mediated epithelial-mesenchymal transition.
| ||
Int J Biol Sci Lactobacillus paracasei ZJUZ2-3 inhibits gastrointestinal tumors via the IAA-induced AHR/MTDH/NF-κB axis. | ||
Mol Ther Role of HCP5-miR-139-RUNX1 Feedback Loop in Regulating Malignant Behavior of Glioma Cells. | ||
J Crohns Colitis MTDH promotes intestinal inflammation by positively regulating TLR signaling. |
Reviews
What customers say
One reviewer (5/5 stars) tested this antibody on HeLa cells for Western Blot and Immunoprecipitation, using dilutions of 1:1000 and 1:2000. They reported it works very well with both room temperature and overnight incubation. IF has not been tested yet. Overall, the feedback is positive regarding performance in WB and IP applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
eman (Verified Customer) (11-26-2020) | this antibody works very well incubating either at room temperature or overnight2 dilutions are tested 1:1000 and 1:2000IF not tested yet
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