MTHFS Polyclonal antibody

MTHFS Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 13114-1-AP

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

Methenyl-THF synthetase, Methenyl THF synthetase, HsT19268, EC:6.3.3.2, 5-formyltetrahydrofolate cyclo-ligase

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Filter: AllWBIPIHC

WB analysis using 13114-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13114-1-AP (MTHFS antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human liver using 13114-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 13114-1-AP (MTHFS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 13114-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13114-1-AP (MTHFS antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Jurkat using 13114-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 13114-1-AP (MTHFS antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human stomach using 13114-1-AP
human stomach tissue were subjected to SDS PAGE followed by western blot with 13114-1-AP (MTHFS antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of U-937 using 13114-1-AP
U-937 cells were subjected to SDS PAGE followed by western blot with 13114-1-AP (MTHFS antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa using 13114-1-AP
IP result of anti-MTHFS (IP:13114-1-AP, 4ug; Detection:13114-1-AP 1:2000) with HeLa cells lysate 1200 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 13114-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 13114-1-AP (MTHFS antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 13114-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 13114-1-AP (MTHFS antibody) at dilution of 1:100 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 13114-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 13114-1-AP (MTHFS antibody) at dilution of 1:100 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 13114-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 13114-1-AP (MTHFS antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols
Reviews (1)

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, human liver tissue, Jurkat cells, human stomach tissue, U-937 cells, L02 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13114-1-AP targets MTHFS in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3781 Product name: Recombinant human MTHFS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-203 aa of BC019921 Sequence: MAAAAVSSAKRSLRGELKQRLRAMSAEERLRQSRVLSQKVIAHSEYQKSKRISIFLSMQDEIETEEIIKDIFQRGKICFIPRYRFQSNHMDMVRIESPEEISLLPKTSWNIPQPGEGDVREEALSTGGLDLIFMPGLGFDKHGNRLGRGKGYYDAYLKRCLQHQEVKPYTLALAFKEQICLQVPVNENDMKVDEVLYEDSSTA Predict reactive species
Full Name	5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase)
Calculated Molecular Weight	203 aa, 23 kDa
Observed Molecular Weight	23 kDa
GenBank Accession Number	BC019921
Gene Symbol	MTHFS
Gene ID (NCBI)	10588
RRID	AB_2250977
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P49914
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for MTHFS antibody 13114-1-AP	Download protocol
IP protocol for MTHFS antibody 13114-1-AP	Download protocol
WB protocol for MTHFS antibody 13114-1-AP	Download protocol