Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, mouse brain tissue, Neuro-2a cells |
| Positive IF/ICC detected in | SH-SY5Y cells |
| Positive FC (Intra) detected in | RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24565-1-AP targets MYO10 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18882 Product name: Recombinant human MYO10 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1669-1917 aa of BC137168 Sequence: ALFTYESLKKTKCREFVPSRDEIEALIHRQEMTSTVYCHGGGSCKITINSHTTAGEVVEKLIRGLAMEDSRNMFALFEYNGHVDKAIESRTVVADVLAKFEKLAATSEVGDLPWKFYFKLYCFLDTDNVPKDSVEFAFMFEQAHEAVIHGHHPAPEENLQVLAALRLQYLQGDYTLHAAIPPLEEVYSLQRLKARISQSTKTFTPCERLEKRRTSFLEGTLRRSFRTGSVVRQKVEEEQMLDMWIKEEV Predict reactive species |
| Full Name | myosin X |
| Calculated Molecular Weight | 2058 aa, 237 kDa |
| Observed Molecular Weight | 240-250 kDa |
| GenBank Accession Number | BC137168 |
| Gene Symbol | MYO10 |
| Gene ID (NCBI) | 4651 |
| RRID | AB_2879611 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HD67 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for MYO10 antibody 24565-1-AP | Download protocol |
| IF protocol for MYO10 antibody 24565-1-AP | Download protocol |
| WB protocol for MYO10 antibody 24565-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MYO10 (Myosin X) antibody (Cat# 24565-1-AP) has 5 total citations published in Science Advances, Proteomics Clinical Applications, PLoS One, Cytoskeleton, and eLife. The associated research fields span cancer genomics and inflammation, ureteropelvic junction obstruction, meiotic spindle assembly, mitotic cell division and cytoskeletal regulation, and sensory axon outgrowth in neural development. Applications include immunofluorescence and Western blot across human, mouse, and zebrafish species.
| Species | Application | Title |
|---|---|---|
Proteomics Clin Appl Quantitative Proteome of Infant Stenotic Ureters Reveals Extracellular Matrix Organization and Oxidative Stress Dysregulation Underlying Ureteropelvic Junction Obstruction. | ||
PLoS One NIMA-related kinase 1 (NEK1) regulates meiosis I spindle assembly by altering the balance between α-Adducin and Myosin X. | ||
Cytoskeleton (Hoboken) The myosin regulatory light chain Myl5 localizes to mitotic spindle poles and is required for proper cell division. | ||
