Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, COS-7 cells |
| Positive IHC detected in | human kidney tissue, rat kidney tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23906-1-AP targets MYO19 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat, monkey samples.
| Tested Reactivity | human, rat, monkey |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20995 Product name: Recombinant human MYO19 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 441-770 aa of BC008900 Sequence: EACGLVETIHISAAGFPIRVSHRNFVERYKLLRRLHPCTSSGPDSPYPAKGLPEWCPHSEEATLEPLIQDILHTLPVLTQAAAITGDSAEAMPAPMHCGRTKVFMTDSMLELLECGRARVLEQCARCIQGGWRRHRHREQERQWRAVMLIQAAIRSWLTRKHIQRLHAAATVIKRAWQKWRIRMACLAAKELDGVEEKHFSQAPCSLSTSPLQTRLLEAIIRLWPLGLVLANTAMGVGSFQRKLVVWACLQLPRGSPSSYTVQTAQDQAGVTSIRALPQGSIKFHCRKSPLRYADICPEPSPYSITGFNQILLERHRLIHVTSSAFTGLG Predict reactive species |
| Full Name | myosin XIX |
| Calculated Molecular Weight | 970 aa, 109 kDa |
| Observed Molecular Weight | 109 kDa |
| GenBank Accession Number | BC008900 |
| Gene Symbol | MYO19 |
| Gene ID (NCBI) | 80179 |
| RRID | AB_2879357 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96H55 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYO19 is also named as MYOHD1. It is Actin-based motor molecule with ATPase activity that localizes to the mitochondrion outer membrane (PubMed:19932026, PubMed:23568824, PubMed:25447992) and required for mitochondrial inheritance during mitosis (PubMed:25447992). MYO19 can enable actin binding activity and be involved in regulation of cytokinesis and regulation of mitochondrial fission. MYO19 can be detected as about 109 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MYO19 antibody 23906-1-AP | Download protocol |
| IHC protocol for MYO19 antibody 23906-1-AP | Download protocol |
| WB protocol for MYO19 antibody 23906-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MYO19 Polyclonal antibody (Cat# 23906-1-AP) has 3 citations published in Scientific Advances, Journal of Pharmaceutical Analysis, and Oncogene. The associated research fields include mitochondrial distribution and inheritance, acute respiratory distress syndrome (ARDS) involving mitochondrial division, and nasopharyngeal carcinoma related to migrasome-mediated mitochondrial transfer. Note: this product has been replaced by Cat# 88684-1-RR.
| Species | Application | Title |
|---|---|---|
Sci Adv ARMC1 partitions between distinct complexes and assembles MIRO with MTFR to control mitochondrial distribution | ||
J Pharm Anal Coral calcium hydride promotes peripheral mitochondrial division and reduces AT-II cells damage in ARDS via activation of the Trx2/Myo19/Drp1 pathway | ||
Oncogene PinX1 inhibits migrasomes-mediated mitochondrial transfer to confer cisplatin sensitivity in nasopharyngeal carcinoma.
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