MSI2 Polyclonal antibody

MSI2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10770-1-AP

Synonyms

MSI2, MSI2H, Musashi 2, musashi homolog 2 (Drosophila)



Tested Applications

Positive WB detected inPC-12 cells, C6 cells, COLO 320 cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman ovary tumor tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10770-1-AP targets MSI2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MSI2 fusion protein Ag1213
Full Name musashi homolog 2 (Drosophila)
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 37-40 kDa
GenBank Accession NumberBC001526
Gene Symbol MSI2
Gene ID (NCBI) 124540
RRIDAB_2147935
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MSI2 is an RNA-binding protein that regulates the expression of target mRNAs at the translation level. It binds to RNA via 2 ribonucleoprotein-type RNA recognition motifs in their N-termini, to consensus motifs in mRNAs to inhibit transcription. It may play a role in the proliferation and maintenance of stem cells in the central nervous system. MSI2 can regulate normal hematopoiesis and promote aggressive myeloid leukemia

Protocols

Product Specific Protocols
WB protocol for MSI2 antibody 10770-1-APDownload protocol
IHC protocol for MSI2 antibody 10770-1-APDownload protocol
IP protocol for MSI2 antibody 10770-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Science

Neurodevelopmental protein Musashi-1 interacts with the Zika genome and promotes viral replication.

Authors - Pavithra L Chavali
humanWB

EMBO J

Suppression of miR-199a maturation by HuR is crucial for hypoxia-induced glycolytic switch in hepatocellular carcinoma.

Authors - Ling-Fei Zhang
humanWB

Int J Oncol

Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells.

Authors - Wei Jia
humanIHC

Mod Pathol

Progenitor stem cell marker expression by pulmonary carcinomas.

Authors - Moreira Andre L AL
humanWB, IP, IF

Front Mol Biosci

RNA-Binding Protein MSI2 Binds to miR-301a-3p and Facilitates Its Distribution in Mitochondria of Endothelial Cells.

Authors - Qian Qian Guo
humanIHC

Exp Cell Res

Msi2 plays a carcinogenic role in esophageal squamous cell carcinoma via regulation of the Wnt/β-catenin and Hedgehog signaling pathways.

Authors - Zhiwei Li