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  • KD/KO Validated

NAP1L4 Polyclonal antibody

NAP1L4 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16018-1-AP

Synonyms

hNAP2, NAP 2, NAP1L4, NAP2, NAP2L, Nucleosome assembly protein 2



Tested Applications

Positive WB detected inHeLa cells, mouse liver tissue, mouse testis tissue, HEK-293 cells, A431 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16018-1-AP targets NAP1L4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NAP1L4 fusion protein Ag8827
Full Name nucleosome assembly protein 1-like 4
Calculated Molecular Weight 375 aa, 43 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC022090
Gene Symbol NAP1L4
Gene ID (NCBI) 4676
RRIDAB_2150711
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NAP1L4 antibody 16018-1-APDownload protocol
IHC protocol for NAP1L4 antibody 16018-1-APDownload protocol
IP protocol for NAP1L4 antibody 16018-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Biol

Phase separation and toxicity of C9orf72 poly(PR) depends on alternate distribution of arginine.

Authors - Chen Chen
humanWB

Mol Cell Proteomics

A novel immuno-competitive capture mass spectrometry strategy for protein-protein interaction profiling reveals that LATS kinases regulate HCV replication through NS5A phosphorylation.

Authors - Hélène Meistermann
mouseIF,WB

J Virol

NAP1L1 and NAP1L4 binding to Hypervariable Domain of Chikungunya Virus nsP3 Protein is bivalent and requires phosphorylation.

Authors - Francisco Dominguez
humanWB,IHC

J Dermatol Sci

Nucleosome assembly protein 1-like 4, a new therapeutic target for proliferation and invasion of melanoma cells.

Authors - Satoru Mizuhashi
  • KD Validated
humanWB

Nat Struct Mol Biol

Mechanism of client selection by the protein quality-control factor UBE2O

Authors - Matthew C J Yip
humanWB

iScience

Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges

Authors - Tamami Miyagi