Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells |
| Positive IHC detected in | human stomach cancer tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23845-1-AP targets NARG1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20842 Product name: Recombinant human NARG1 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-350 aa of BC093928 Sequence: MPAVSLPPKENALFKRILRCYEHKQYRNGLKFCKQILSNPKFAEHGETLAMKGLTLNCLGKKEEAYELVRRGLRNDLKSHVCWHVYGLLQRSDKKYDEAIKCYRNALKWDKDNLQILRDLSLLQIQMRDLEGYRETRYQLLQLRPAQRASWIGYAIAYHLLEDYEMAAKILEEFRKTQQTSPDKVDYEYSELLLYQNQVLREAGLYREALEHLCTYEKQICDKLAVEETKGELLLQLCRLEDAADVYRGLQERNPENWAYYKGLEKALKPANMLERLKIYEEAWTKYPRGLVPRRLPLNFLSGEKFKECLDKFLRMNFSKGCPPVFNTLRSLYKDKEKVAIIEELVVGYE Predict reactive species |
| Full Name | NMDA receptor regulated 1 |
| Calculated Molecular Weight | 866 aa, 101 kDa |
| Observed Molecular Weight | 95-100 kDa |
| GenBank Accession Number | BC093928 |
| Gene Symbol | NARG1 |
| Gene ID (NCBI) | 80155 |
| RRID | AB_2879338 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q9BXJ9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NAA15 (N-alpha-acetyltransferase 15) is a subunit of both the NatA and NatE complexes, and its role is to position the catalytic subunits in close vicinity to the nascent polypeptides (PMID: 33557580). Human NAA15 encodes an 866 amino acid (~105 kDa) protein, NAA15, containing tetratricopeptide repeat domains and a putative bipartite nuclear localization signal (PMID: 29656860). NAA15 was implicated in human disease and loss-of-function NAA15 variants in large cohorts of patients with autism spectrum disorder and/or ID (PMID: 33103328).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NARG1 antibody 23845-1-AP | Download protocol |
| IHC protocol for NARG1 antibody 23845-1-AP | Download protocol |
| WB protocol for NARG1 antibody 23845-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Morgane (Verified Customer) (10-01-2025) | Migrates at expected size and clean signal
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FH Zehua (Verified Customer) (07-09-2018) | Samples from ProteinTech. Works very well.
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