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WB analysis using 13187-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13187-1-AP (Neurochondrin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 13187-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 13187-1-AP (Neurochondrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 13187-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 13187-1-AP (Neurochondrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 13187-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 13187-1-AP (Neurochondrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 13187-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13187-1-AP (Neurochondrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 13187-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13187-1-AP (Neurochondrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 13187-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13187-1-AP (Neurochondrin antibody at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 13187-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13187-1-AP (Neurochondrin antibody at dilution of 1:50 (under 40x lens).

Neurochondrin Polyclonal antibody

Name: Neurochondrin Polyclonal antibody
Brand: Proteintech
SKU: 13187-1-AP

Neurochondrin Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13187-1-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Synonyms

KIAA0607, NCDN, Neurochondrin

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse brain tissue, human brain tissue, rat brain tissue
Positive IHC detected in	mouse brain tissue, human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IF	See 1 publications below

Product Information

13187-1-AP targets Neurochondrin in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3859 Product name: Recombinant human NCDN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 426-729 aa of BC024592 Sequence: KTLYEEAEEANDLSQQVANLAISPTTPGPTWPGDALRLLLPGWCHLTVEDGPREILIKEGAPSLLCKYFLQQWELTSPGHDTSVLPDSVEIGLQTCCHIFLNLVVTAPGLIKRDACFTSLMNTLMTSLPALVQQQGRLLLAANVATLGLLMARLLSTSPALQGTPASRGFFAAAILFLSQSHVARATPGSDQAVLALSPEYEGIWADLQELWFLGMQAFTGCVPLLPWLAPAALRSRWPQELLQLLGSVSPNSVKPEMVAAYQGVLVELARANRLCREAMRLQAGEETASHYRMAALEQCLSEP Predict reactive species
Full Name	neurochondrin
Calculated Molecular Weight	729 aa, 79 kDa
Observed Molecular Weight	79 kDa
GenBank Accession Number	BC024592
Gene Symbol	Neurochondrin
Gene ID (NCBI)	23154
RRID	AB_2877919
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UBB6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Neurochondrin antibody 13187-1-AP	Download protocol
IHC protocol for Neurochondrin antibody 13187-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB,IF	J Cell Sci Neurochondrin interacts with the SMN protein suggesting a novel mechanism for spinal muscular atrophy pathology. Authors - Luke W Thompson View Article