Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17262-1-AP targets NDEL1 in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10975 Product name: Recombinant human NDEL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-345 aa of BC026101 Sequence: MDGEDIPDFSSLKEETAYWKELSLKYKQSFQEARDELVEFQEGSRELEAELEAQLVQAEQRNRDLQADNQRLKYEVEALKEKLEHQYAQSYKQVSVLEDDLSQTRAIKEQLHKYVRELEQANDDLERAKRATIVSLEDFEQRLNQAIERNAFLESELDEKESLLVSVQRLKDEARDLRQELAVRERQQEVTRKSAPSSPTLDCEKMDSAVQASLSLPATPVGKGTENTFPSPKAIPNGFGTSPLTPSARISALNIVGDLLRKVGALESKLAACRNFAKDQASRKSYISGNVNCGVLNGNGTKFSRSGHTSFFDKGAVNGFDTAPPPPGLGSSRPSSAPGMLPLSV Predict reactive species |
| Full Name | nudE nuclear distribution gene E homolog (A. nidulans)-like 1 |
| Calculated Molecular Weight | 345 aa, 38 kDa, 41 kDa |
| Observed Molecular Weight | 38-42 kDa |
| GenBank Accession Number | BC026101 |
| Gene Symbol | NDEL1 |
| Gene ID (NCBI) | 81565 |
| RRID | AB_2235821 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9GZM8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Nuclear distribution factor E-homolog 1 (NDE1), NDE-like 1 (NDEL1), and Lissencephaly 1 (LIS1) together involve in essential neurodevelopmental processes, including neuronal precursor proliferation and differentiation, neurite outgrowth, and neuronal migration. All three bind directly to Disrupted in Schizophrenia 1 (DISC1), a scaffold protein critical to neuronal proliferation, integration, migration, and synaptic function within the developing and adult brain.[PMID: 18469341] Nuclear distribution element-like 1 (NDEL1) was firstly identified as a regulator of the cytoskeleton in microtubule and intermediate fliament dynamics and microtubule-based transport [PMID:21948775]. It is required for organization of the cellular microtubule array and microtubule anchoring at the centrosome, which is in part by targeting the microtubule severing protein KATNA1 to the centrosome[PMID:15728732].
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for NDEL1 antibody 17262-1-AP | Download protocol |
| WB protocol for NDEL1 antibody 17262-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NDEL1 Polyclonal antibody (Cat# 17262-1-AP) has 7 citations published in Nature Communications, PNAS, Annals of Neurology, Journal of Cell Biology, Scientific Reports, Disease Models & Mechanisms, and eLife. The research fields span neurodevelopment, microtubule/cytoskeleton dynamics, neuronal migration and morphogenesis, ciliopathy, Zika virus–induced neurological damage, cell migration, and axonal trafficking.
| Species | Application | Title |
|---|---|---|
Nat Commun DISC1 Protects Against Zika Virus Infection and Long-Term Neurological Damage Through AMPK-mTOR-Mediated Autophagy | ||
Proc Natl Acad Sci U S A Gcap14 is a microtubule plus-end-tracking protein coordinating microtubule-actin crosstalk during neurodevelopment | ||
Ann Neurol Extraciliary roles of the ciliopathy protein JBTS17 in mitosis and neurogenesis. | ||
J Cell Biol Lis1 is essential for cortical microtubule organization and desmosome stability in the epidermis. | ||
Sci Rep Regulation of the actin cytoskeleton by the Ndel1-Tara complex is critical for cell migration. | ||
Dis Model Mech 14-3-3θ phosphorylation at S232 reduces its interactome and regulates axonal trafficking.
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