NDOR1/NR1 Polyclonal antibody

NDOR1/NR1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11404-1-AP

Synonyms

NDOR1, NDOR1/NR1, Novel reductase 1, NR1



Tested Applications

Positive WB detected inHaCaT cells, human liver tissue, human spleen tissue, human lung tissue, mouse thymus tissue, mouse testis tissue, HeLa cells, Jurkat cells, U-251 cells, NIH/3T3 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11404-1-AP targets NDOR1/NR1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDOR1/NR1 fusion protein Ag1909
Full Name NADPH dependent diflavin oxidoreductase 1
Calculated Molecular Weight 606 aa, 68 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC015735
Gene Symbol NDOR1
Gene ID (NCBI) 27158
RRIDAB_2251225
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NDOR1/NR1 antibody 11404-1-APDownload protocol
IHC protocol for NDOR1/NR1 antibody 11404-1-APDownload protocol
IF protocol for NDOR1/NR1 antibody 11404-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Life Sci

Geniposide improves depression-like behavior in prenatal stress male offspring through restoring HPA axis- and glucocorticoid receptor-associated dysfunction

Authors - Yu Ma
mouseWB

ACS Chem Neurosci

Antidepressant-like Effect of Merazin Hydrate Depends on NO/ERK by Suppressing Its Downstream NF-κB or Nonactivating CREB/BDNF in Mouse Hippocampus.

Authors - Lei Wu
mouseWB

Phytother Res

Rapid antidepressant-like effect of Fructus Aurantii depends on cAMP-response element binding protein/Brain-derived neurotrophic facto by mediating synaptic transmission.

Authors - Lei Wu
mouseWB

Toxicology

PMID: 29800586

Authors - Xiaolong Wang
mouseWB

Behav Brain Res

Down-regulation of NR2B receptors contributes to the analgesic and antianxiety effects of enriched environment mediated by endocannabinoid system in the inflammatory pain mice

Authors - Shukun Jiang
humanWB

J Mol Neurosci

PMID: 28730337

Authors - Xiaolong Wang