NFE2 Antibody
Rabbit Polyclonal
Catalog number: 11089-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | WB |
| Species specificity | human; other species not tested. |
| Cited species | human |
| Positive WB detected in | K-562 cells |
| Positive IHC detected in | human colon cancer |
| Positive IF detected in | HepG2 cells |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:20-1:200 IF: 1:50-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
K-562 cells were subjected to SDS PAGE followed by western blot with 11089-1-AP(NFE2 Antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human colon cancer using 11089-1-AP(NFE2 Antibody) at Dilution 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using NFE2 antibody 11089-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | NFE2 fusion protein ag1570 | Full name | nuclear factor (erythroid-derived 2), 45kDa |
|
Calculated molecular weight |
41kd |
Observed molecular
weight |
45 kDa |
|
GenBank accession number |
BC005044 | Gene ID (NCBI) | 4778 |
| Gene symbol | NFE2 | Synonyms | NF-E2; p45 |
Background
The transcription factor p45 nuclear factor-erythroid−derived 2(NFE2) is one component of the NF-E2 complex that essential for regulating reythroid and megakaryocytic maturation and differentiation. It also plays crucial roles in erythroid and megakaryocytic lineages. NFE2 recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters, and acts as a activator. And it may has a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron. This is a rabbit polyclonal antibody raised against full-length NFE2 of human origin |
