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NFIA Polyclonal antibody

NFIA Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11750-1-AP

Synonyms

CTF, KIAA1439, NF I/A, NF1 A, NFI A, NFI L, NFIA, Nuclear factor 1 A type, Nuclear factor 1/A, nuclear factor I/A, TGGCA binding protein

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using NFIA Polyclonal antibody (11750-1-AP)

    WB analysis of A431 using 11750-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 11750-1-AP (NFIA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using NFIA Polyclonal antibody (11750-1-AP)

    WB analysis of L02 using 11750-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 11750-1-AP (NFIA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using NFIA Polyclonal antibody (11750-1-AP)

    WB analysis of mouse liver using 11750-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 11750-1-AP (NFIA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NFIA Polyclonal antibody (11750-1-AP)

    WB analysis of HeLa using 11750-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11750-1-AP (NFIA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using NFIA Polyclonal antibody (11750-1-AP)

    WB analysis of Jurkat using 11750-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11750-1-AP (NFIA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A431 cells using NFIA Polyclonal antibody (11750-1-AP)

    IP experiment of A431 using 11750-1-AP

    IP result of anti-NFIA (IP:11750-1-AP, 4ug; Detection:11750-1-AP 1:500) with A431 cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NFIA Polyclonal antibody (11750-1-AP)

    IHC staining of mouse brain using 11750-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11750-1-AP (NFIA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NFIA Polyclonal antibody (11750-1-AP)

    IHC staining of human prostate cancer using 11750-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11750-1-AP (NFIA antibody) at dilution of 1:25 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using NFIA Polyclonal antibody (11750-1-AP)

    IHC staining of human gliomas using 11750-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11750-1-AP (NFIA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using NFIA Polyclonal antibody (11750-1-AP)

    IHC staining of human gliomas using 11750-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11750-1-AP (NFIA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NFIA Polyclonal antibody (11750-1-AP)

    IHC staining of mouse brain using 11750-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11750-1-AP (NFIA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA431 cells, HeLa cells, Jurkat cells, L02 cells, mouse liver tissue
Positive IP detected inA431 cells
Positive IHC detected inmouse brain tissue, human prostate cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11750-1-AP targets NFIA in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NFIA fusion protein Ag2346
Full Name nuclear factor I/A
Calculated Molecular Weight 498 aa, 55 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC022264
Gene Symbol NFIA
Gene ID (NCBI) 4774
RRIDAB_2153214
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The NFI (nuclear factor I) family consists of four members in vertebrates (NFI-A, NFI-B, NFI-C and NFI-X), and the four NFI genes are expressed in unique patterns during mouse embryogenesis and in the adult. Four isoforms of NFIA were found in human and they play various roles in DNA replication, DNA-dependent transcritpion via their DNA binding property. Multiple residues of NFIA can be phosphorylated resulting in mild shifts of its practical molecular weight. Recent finding also revealed its neuroprotective function in NMDA-induced neuronal damage. Catalog# 11750-1-AP is a rabbit polyclonal antibody raised against N-terminal of human original NFIA. The calculated molecular weight of NFIA is 55 kDa, However the size of the proteins cross-linked to the adenoviral NF-I element ranged from 60 to 80 kDa . The larger size observed by us could be due to the oligo protein complex, which would increase the size by 15-20 kDa. (PMID: 11447215)

Protocols

Product Specific Protocols
WB protocol for NFIA antibody 11750-1-APDownload protocol
IHC protocol for NFIA antibody 11750-1-APDownload protocol
IP protocol for NFIA antibody 11750-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Cycle

TRIM71 binds to IMP1 and is capable of positive and negative regulation of target RNAs.

Authors - Daniel J Foster
View Article
humanWB

Cancer Cell Int

Macrophages-derived exosomal lncRNA LIFR-AS1 promotes osteosarcoma cell progression via miR-29a/NFIA axis.

Authors - Hongliang Zhang
View Article
mouseIHC

FEBS Lett

STAT3 transcriptionally regulates the expression of genes related to glycogen metabolism in developing motor neurons

Authors - Hitoshi Gotoh
View Article
humanWB,CoIP

Pathol Res Pract

SOX9/NFIA promotes human ovarian cancer metastasis through the Wnt/β-catenin signaling pathway

Authors - Rong Lu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

KUN (Verified Customer) (01-13-2021)

Works for western blot, recogizning both NFIA and also other NFI family proteins

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: 293T