Tested Applications
| Positive WB detected in | HeLa cells, mouse brain tissue, mouse lung tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21777-1-AP targets TTF1/NKX2-1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | NK2 homeobox 1 |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | NM_003317 |
| Gene Symbol | TTF1 |
| Gene ID (NCBI) | 7080 |
| RRID | AB_2878919 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P43699 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NKX2-1, also named as NKX2A, TITF1 and TTF1, belongs to the NK-2 homeobox family. It is a transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. NKX2-1 is crucial in the maintenance of the thyroid differentiation phenotype. It may play a role in lung development and surfactant homeostasis. Defects in NKX2-1 are the cause of benign hereditary chorea (BHC), choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD). The antibody is specific to NKX2-1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TTF1/NKX2-1 antibody 21777-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Environ Toxicol Pharmacol Role of estrogen receptors in thyroid toxicity induced by mono (2-ethylhexyl) phthalate via endoplasmic reticulum stress: An in vitro mechanistic investigation | ||
Environ Pollut PFOA substitute GenX at a sub-cytotoxic level exhibits thyroid-disrupting effects by inhibiting the expression of Thyroid Transcription Factor 1 | ||
Cancer Res Loss of CDKN2A Enhances the Efficacy of Immunotherapy in EGFR Mutant Non-Small Cell Lung Cancer | ||
Toxicol Appl Pharmacol Prenatal amoxicillin exposure impairs mouse alveolar development via Tet3-mediated hypo-hydroxymethylation of Nkx2-1 promoter. | ||
Phytother Res Chlorogenic Acid Regulates Macrophage Polarization in LUAD by Inhibiting Taurodeoxycholic Acid Metabolism to Block the TGR5/STAT3 Signaling Pathway. | ||
J Genet Genomics Tapt1 deficiency in mice impairs pulmonary lipid homeostasis and normal postnatal respiration by targeting ABCA3 for autophagy-lysosomal degradation. |
