NMD3 Polyclonal antibody
NMD3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 16060-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, L02 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human breast cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 1 publications below |
Product Information
16060-1-AP targets NMD3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NMD3 fusion protein Ag8894 |
| Full Name | NMD3 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 503 aa, 58 kDa |
| Observed Molecular Weight | 58 kDa |
| GenBank Accession Number | BC013317 |
| Gene Symbol | NMD3 |
| Gene ID (NCBI) | 51068 |
| RRID | AB_2282830 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Human NMD3 gene encodes 60S ribosomal export protein which was found in a 60S ribosomal subunit export complex with RAN and XPO1. This nucleocytoplasmic shuttling protein NMD3 is an adaptor for export of the 60S ribosomal subunit from the nucleus. NMD3 contains a CRM-1-dependent leucine-rich nuclear export signal (NES) and a dispersed nuclear localization signal (NLS), the basic region of which is also required for nucleolar accumulation. NMD3 are required for nuclear export of the 60S ribosomal subunit in yeast and vertebrate cells, recent finding has also revealed its role in Arabidopsis thaliana.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NMD3 antibody 16060-1-AP | Download protocol |
| IHC protocol for NMD3 antibody 16060-1-AP | Download protocol |
| IF protocol for NMD3 antibody 16060-1-AP | Download protocol |
| IP protocol for NMD3 antibody 16060-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Stem Cell HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis. | ||
Genes Dev Uncoupling of GTP hydrolysis from eIF6 release on the ribosome causes Shwachman-Diamond syndrome. | ||
Nucleic Acids Res The induction of p53 correlates with defects in the production, but not the levels, of the small ribosomal subunit and stalled large ribosomal subunit biogenesis | ||
Cancer Lett RNA-binding protein hnRNPU regulates multiple myeloma resistance to selinexor |
