Tested Applications
| Positive WB detected in | mouse kidney tissue, mouse skeletal muscle tissue |
| Positive IF-P detected in | mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13236-1-AP targets NMNAT3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3965 Product name: Recombinant human NMNAT3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-215 aa of BC034374 Sequence: MYQVIQGIISPVNDTYGKKDLAASHHRVAMARLALQTSDWIRVDPWESEQAQWMETVKVLRHHHSKLLRSPPQMEGPDHGKALFSTPAAVPELKLLCGADVLKTFQTPNLWKDAHIQEIVEKFGLVCVGRVGHDPKGYIAESPILRMHQHNIHLAKEPVQNEISATYIRRALGQGQSVKYLIPDAVITYIKDHGLYTKGSTWKGKSTQSTEGKTS Predict reactive species |
| Full Name | nicotinamide nucleotide adenylyltransferase 3 |
| Calculated Molecular Weight | 215 aa, 24 kDa |
| Observed Molecular Weight | 24-28 kDa |
| GenBank Accession Number | BC034374 |
| Gene Symbol | NMNAT3 |
| Gene ID (NCBI) | 349565 |
| RRID | AB_2877927 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96T66 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NMNAT3 antibody 13236-1-AP | Download protocol |
| WB protocol for NMNAT3 antibody 13236-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NMNAT3 Polyclonal antibody (Cat# 13236-1-AP) has 6 total citations published in Nature Cardiovascular Research, Biosensors and Bioelectronics, International Journal of Biological Sciences, EMBO Journal, Food & Function, and International Journal of Molecular Sciences. The cited research spans cardiovascular disease (aortic aneurysm), NAD⁺ metabolism and homeostasis, neurodegeneration (ALS), cardiac function, aging and colon health, and diabetic nephropathy. Applications include WB and IHC in human, mouse, and rat samples.
| Species | Application | Title |
|---|---|---|
Nat Cardiovasc Res Mitochondrial NAD+ deficiency in vascular smooth muscle impairs collagen III turnover to trigger thoracic and abdominal aortic aneurysm | ||
Biosens Bioelectron Quantitative dynamics of intracellular NMN by genetically encoded biosensor | ||
Int J Biol Sci Nicotinamide Riboside Enhances Mitochondrial Proteostasis and Adult Neurogenesis through Activation of Mitochondrial Unfolded Protein Response Signaling in the Brain of ALS SOD1G93A Mice. | ||
EMBO J Control of NAD+ homeostasis by autophagic flux modulates mitochondrial and cardiac function | ||
Food Funct β-Nicotinamide mononucleotide supplementation prolongs the lifespan of prematurely aged mice and protects colon function in ageing mice | ||
Int J Mol Sci Nmnat1 Deficiency Causes Mitoribosome Excess in Diabetic Nephropathy Mediated by Transcriptional Repressor HIC1 |
Reviews
What customers say
One reviewer used this antibody for Western Blot on mouse DRG neuronal lysate at a 1:300 dilution. The antibody successfully detected the target protein, though the bands were described as very faint, which the user attributed to low protein concentration in their sample rather than an issue with the antibody itself. Overall rating was 4 out of 5.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Caitlin (Verified Customer) (01-09-2020) | I used the NMNAT3 antibody for protein detection via Western Blot on mouse DRG neuronal lysate. The antibody was able to detect my protein of interest, but the bands were very faint (likely due to low protein concentration on my end).
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