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- KD/KO Validated
NR1H2 Monoclonal antibody
NR1H2 Monoclonal Antibody for WB, FC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, pig and More (1)
Applications
WB, FC, IHC, ChIP, ELISA
Conjugate
Unconjugated
CloneNo.
1E12B10
Cat no : 60345-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human liver tissue, pig liver tissue |
| Positive FC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 11 publications below |
| IHC | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
60345-1-Ig targets NR1H2 in WB, FC, IHC, ChIP, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | NR1H2 fusion protein Ag5635 |
| Full Name | nuclear receptor subfamily 1, group H, member 2 |
| Calculated Molecular Weight | 51 kDa |
| Observed Molecular Weight | 51 kDa |
| GenBank Accession Number | BC047750 |
| Gene Symbol | NR1H2 |
| Gene ID (NCBI) | 7376 |
| RRID | AB_2881454 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NR1H2, also known as LXRB, is a liver X receptors, which form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. It preferentially bind to double-stranded oligonucleotide direct repeats, which have the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). It involves in the regulation of cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NR1H2 antibody 60345-1-Ig | Download protocol |
| FC protocol for NR1H2 antibody 60345-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Br J Pharmacol Functional interplay between LXR and AMPKα inhibits atherosclerosis in apoE deficient mice - A new anti-atherogenic strategy. | ||
Front Oncol EGFR/FOXO3A/LXR-α Axis Promotes Prostate Cancer Proliferation and Metastasis and Dual-Targeting LXR-α/EGFR Shows Synthetic Lethality. | ||
J Lipid Res 25-Hydroxycholesterol activates the expression of cholesterol 25-hydroxylase in an LXR-dependent mechanism.
| ||
J Lipid Res Activation of liver X receptor plays a central role in antiviral actions of 25-hydroxycholesterol.
| ||
J Biol Chem TL1A inhibits atherosclerosis in apoE-deficient mice by regulating the phenotype of vascular smooth muscle cells. | ||
J Agric Food Chem Diosmetin Ameliorates Nonalcoholic Steatohepatitis through Modulating Lipogenesis and Inflammatory Response in a STAT1/CXCL10-Dependent Manner. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Zehua (Verified Customer) (11-07-2019) | This antibody works well in liver lysates from wild-type and hLXR knockin mouse.
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