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  • Featured Product
  • KD/KO Validated

PXR Polyclonal antibody

PXR Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 15607-1-AP

Synonyms

BXR, NR1I2, ONR1, Orphan nuclear receptor PAR1, Orphan nuclear receptor PXR, PAR, PAR1, PAR2, PARq, Pregnane X receptor, PRR, PXR, SAR, SXR

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PXR Polyclonal antibody (15607-1-AP)

    WB analysis of MCF-7 using 15607-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15607-1-AP (NR1I2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using PXR Polyclonal antibody (15607-1-AP)

    WB analysis of A549 using 15607-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 15607-1-AP (NR1I2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PXR Polyclonal antibody (15607-1-AP)

    WB analysis of HepG2 using 15607-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15607-1-AP (NR1I2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using PXR Polyclonal antibody (15607-1-AP)

    WB analysis of mouse colon using 15607-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 15607-1-AP (NR1I2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15607-1-AP targets PXR in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PXR fusion protein Ag7991
Full Name nuclear receptor subfamily 1, group I, member 2
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC017304
Gene Symbol NR1I2
Gene ID (NCBI) 8856
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pregnane X receptor (PXR; NR1I2), a member of the nuclear receptor superfamily, plays a key role in the induction of genes involved in drug transport and metabolism. It modulates drug transport and metabolism through the regulation of target genes responsible for the transport and conversion of chemicals into metabolites that are more easily eliminated from the body [PMID:22609277]. It is also emerging as an endobiotic receptor that regulates the inflammatory response. In addition, PXR acts as a transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds [PMID: 19297428].

Publications

SpeciesApplicationTitle
humanIF,WB

Hepatol Int

Hepatitis B virus-related intrahepatic cholangiocarcinoma originates from hepatocytes

Authors - Zimin Song
View Article
mouseCoIP

Cell Death Dis

Poly(ADP-ribosyl)ated PXR is a critical regulator of acetaminophen-induced hepatotoxicity.

Authors - Cheng Wang
  • KD Validated
View Article
humanWB

Biochem Pharmacol

Constitutive androstane receptor weakens the induction of panaxytriol on CYP3A4 by repressing the activation of pregnane X receptor.

Authors - Qingqing Hu
View Article
mouseWB

Br J Nutr

Retinoic acid induces macrophage cholesterol efflux and inhibits atherosclerotic plaque formation in apoE-deficient mice.

Authors - Wenjing Zhou
View Article
humanWB,IHC

Ann Transl Med

Curcuma zedoaria petroleum ether extract reverses the resistance of triple-negative breast cancer to docetaxel via pregnane X receptor.

Authors - En-Qi Qiao
View Article
humanIHC

Reprod Sci

Protease Amplification of the Inflammatory Response Induced by Commensal Bacteria: Implications for Racial Disparity in Term and Preterm Birth.

Authors - Scott W Walsh
View Article