Tested Applications
| Positive IHC detected in | human retinoblastoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 2 publications below |
Product Information
17388-1-AP targets NRL in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11277 Product name: Recombinant human NRL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-237 aa of BC012395 Sequence: MALPPSPLAMEYVNDFDLMKFEVKREPSEGRPGPPTASLGSTPYSSVPPSPTFSEPGMVGATEGTRPGLEELYWLATLQQQLGAGEALGLSPEEAMELLQGQGPVPVDGPHGYYPGSPEETGAQHVQLAERFSDAALVSMSVRELNRQLRGCGRDEALRLKQRRRTLKNRGYAQACRSKRLQQRRGLEAERARLAAQLDALRAEVARLARERDLYKARCDRLTSSGPGSGDPSHLFL Predict reactive species |
| Full Name | neural retina leucine zipper |
| Calculated Molecular Weight | 237 aa, 26 kDa |
| GenBank Accession Number | BC012395 |
| Gene Symbol | NRL |
| Gene ID (NCBI) | 4901 |
| RRID | AB_2878400 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P54845 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NRL antibody 17388-1-AP | Download protocol |
| WB protocol for NRL antibody 17388-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Human brain organoids assemble functionally integrated bilateral optic vesicles. | ||
JCI Insight All-in-one AAV-mediated Nrl gene inactivation rescues retinal degeneration in Pde6a mice | ||
Nat Protoc Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Zhiquan (Verified Customer) (01-12-2024) | This anti-NRL antibody works well for WB in P30 Mouse retina.
 |
FH Alessandro (Verified Customer) (01-19-2023) | great outcome, no unspecific staining
|
